A labelled-ubiquicidin antimicrobial peptide for immediate in situ optical detection of live bacteria in human alveolar lung tissue

被引：77

作者：

Akram, Ahsan R. ^{[1
]}

Avlonitis, Nicolaos ^{[2
]}

Lilienkampf, Annamaria ^{[2
]}

Perez-Lopez, Ana M. ^{[2
]}

McDonald, Neil ^{[1
]}

Chankeshwara, Sunay V. ^{[2
]}

Scholefield, Emma ^{[1
]}

Haslett, Christopher ^{[1
]}

Bradley, Mark ^{[2
]}

Dhaliwal, Kevin ^{[1
]}

机构：

[1] Queens Med Res Inst, MRC Ctr Inflammat Res, Pulm Opt Mol Imaging Grp, Edinburgh EH16 4TJ, Midlothian, Scotland

[2] Univ Edinburgh, Sch Chem, EaStCHEM, Edinburgh EH9 3FJ, Midlothian, Scotland

来源：

CHEMICAL SCIENCE | 2015年 / 6卷 / 12期

基金：

英国惠康基金; 英国工程与自然科学研究理事会;

关键词：

VIVO; INFLAMMATION; CYCLIZATION; PNEUMONIA;

D O I：

10.1039/c5sc00960j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The in situ immediate detection of the presence of bacteria in the distal human lung is of significant clinical utility. Herein we describe the development and optimization of a bacterial binding fragment (UBI29-41) of the antimicrobial peptide, ubiquicidin (UBI), conjugated to an environmentally sensitive fluorophore to enable rapid live bacterial imaging within human lung tissue. UBI29-41 was modified for stability in the presence of human lung bronchoalveolar lavage fluid, for affinity to bacterial membranes and functionality in human lung tissue. The optimized cyclic structure yields an optical molecular Smartprobe for bacterial detection in human lung tissue.

引用

页码：6971 / 6979

页数：9

共 27 条

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