Examination of OPG, RANK, RANKL and HIF1A polymorphisms in temporomandibular joint ankylosis patients

Purpose: To evaluate the association between polymorphisms in genes that regulate bone metabolism, such as OPG, RANK, RANKL, and HIFIA, in patients with temporomandibular joint (TMJ) ankylosis. Methods: The sample consisted of 181 individuals, the study included 17 individuals with TMJ ankylosis and 164 controls. DNA was extracted from buccal epithelial cells. The genotyping of genetic poly-morphisms in OPG (rs2073618), RANK (rs3826620), RANKL (rs9594738), and HIFIA (rs2301113 and rs2057482) was performed by real-time PCR using TaqMan (TM) technology (Applied Biosystems). The data were subjected to statistical analysis with a level of significance of 0.05. Results: The OPG (rs2073618) polymorphism was associated with TMJ ankylosis, both in the additive model and in the dominant model (p < 0.05). In the additive model, when the individuals carried the CC genotype, they presented as 10.80 times more likely to develop the condition (p = 0.03). In the dominant model, individuals that carried at least one C allele were 5.76 times more likely to have TMJ ankylosis, than those with the G allele (p = 0.01). Conclusion: The polymorphism rs2073618 of OPG is a possible marker that is associated with the risk of manifestation of TMJ ankylosis. (C) 2019 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.