Determination of phenol and catechol in tobacco leaf by solid phase extraction and high performance liquid chromatography

被引：0

作者：

Li, Z ^{[1
]}

Wang, L ^{[1
]}

Yang, GY ^{[1
]}

Liu, W ^{[1
]}

Shi, GL ^{[1
]}

Jiang, CQ ^{[1
]}

机构：

[1] Yunnan Acad Tobacco Sci, Chem Lab, Kunming 650106, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 29卷 / 12期

关键词：

high performance liquid chromatography; phenol; catechol; tobacco leaf;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the determination of phenol and catechol in tobacco leaf by high performance liquid chromatography with methanol and 0.05 mol/L of monopotassium phosphate as mobile phase at a flow-rate of 0.5 mL/min was described. Phenol and catechol were extracted from tobacco sample by distillation and solid phase extraction with Sep-Park-C-18 cartridge. The analytical column is Nova-Pak-C-18 (3.9 x 150 nun). The detection wavelengths of phenol and catechol are UV-270.3 nm, and UV-275.0 nm respectively. The recoveries of phenol and catechol. are 91.8% similar to 104.5% and 89.6% similar to 103.8% respectively. This method has been applied to the determination of phenol and catechol in tobacco with satisfactory result.

引用

页码：1409 / 1411

页数：3