Melatonin suppresses apoptosis and stimulates progesterone production by bovine granulosa cells via its receptors (MT1 and MT2)

被引:97
作者
Wang, S. -J. [1 ]
Liu, W. -J. [1 ]
Wu, C. -J. [1 ]
Ma, F. -H. [1 ]
Ahmad, S. [1 ]
Liu, B. -R. [1 ]
Han, L. [1 ]
Jiang, X. -P. [1 ]
Zhang, S. -J. [1 ]
Yang, L. -G. [1 ]
机构
[1] Huazhong Agr Univ, Minist Educ, Key Lab Agr Anim Genet Breeding & Reprod, Wuhan, Hubei, Peoples R China
关键词
Melatonin; Granulosa cell; Apoptosis; Proliferation; Progesterone; Estradiol; STEROID-PRODUCTION; INVITRO SECRETION; SEASONAL RHYTHMS; MESSENGER-RNA; RAT-BRAIN; IN-VITRO; EXPRESSION; OOCYTE; MITOCHONDRIAL; MATURATION;
D O I
10.1016/j.theriogenology.2012.06.019
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Melatonin and its receptors have been detected in the ovary of many species, and mediate ovarian functions. The present study was designed to investigate the expression and subcellar location of melatonin receptors in bovine granulosa cells (GCs), using reverse transcription (RT) polymerase chain reaction, Western blot, and immunofluorescence analyses. Furthermore, expression level of melatonin receptors rnRNA (real-time polymerase chain reaction) after treatment with various concentrations of melatonin, as well as its effects on cell apoptosis, proliferation, and steroidogenesis (by flow cytometry and RIA), were determined. In bovine GCs, melatonin receptors MT1 and MT2 were differentially located at the cell membrane, the cytoplasm, and nuclear membranes. The expression of MT1 and MT2 mRNA was regulated differently by melatonin in time- and dose-dependent manners. Exogenous melatonin suppressed cell apoptosis (P < 0.05) but not proliferation (P > 0.05). After 72 h, the apoptotic rate was significantly inhibited in all treatment groups. Meanwhile, melatonin supplementation stimulated progesterone production, but inhibited estradiol biosynthesis, in a time-dependent manner. Progesterone production was highest (P < 0.05) at 72 h. Estradiol concentrations were almost unaffected (P > 0.05) at 24 h, but were decreased (P < 0.05) at 48 h. In conclusion, exogenous melatonin acts via receptors and has important roles in regulation of development and function of bovine GCs. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:1517 / 1526
页数:10
相关论文
共 37 条
  • [1] Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis
    Adriaens, I.
    Jacquet, P.
    Cortvrindt, R.
    Janssen, K.
    Smitz, J.
    [J]. TOXICOLOGY, 2006, 228 (2-3) : 333 - 343
  • [2] EFFECT OF MELATONIN ON THE INVITRO SECRETION OF PROGESTERONE AND ESTRADIOL 17-BETA BY OVINE GRANULOSA-CELLS
    BARATTA, M
    TAMANINI, C
    [J]. ACTA ENDOCRINOLOGICA, 1992, 127 (04): : 366 - 370
  • [3] Digging deep - structure-function relationships in the melatonin receptor family
    Barrett, P
    Conway, S
    Morgan, PJ
    [J]. JOURNAL OF PINEAL RESEARCH, 2003, 35 (04) : 221 - 230
  • [4] Exogenous melatonin positively influences follicular dynamics, oocyte developmental competence and blastocyst output in a goat model
    Berlinguer, Fiammetta
    Leoni, Giovanni G.
    Succu, Sara
    Spezzigu, Antonio
    Madeddu, Manuela
    Satta, Valentina
    Bebbere, Daniela
    Contreras-Solis, Ignacio
    Gonzalez-Bulnes, Antonio
    Naitana, Salvatore
    [J]. JOURNAL OF PINEAL RESEARCH, 2009, 46 (04) : 383 - 391
  • [5] PINEAL MELATONIN IN SYRIAN-HAMSTERS - CIRCADIAN AND SEASONAL RHYTHMS IN ANIMALS MAINTAINED UNDER LABORATORY AND NATURAL CONDITIONS
    BRAINARD, GC
    PETTERBORG, LJ
    RICHARDSON, BA
    REITER, RJ
    [J]. NEUROENDOCRINOLOGY, 1982, 35 (05) : 342 - 348
  • [6] Channing C P, 1975, Methods Enzymol, V39, P183
  • [7] CIRCADIAN AND SEASONAL RHYTHMS OF MELATONIN PRODUCTION IN MULES (EQUUS-ASINUS X EQUUS-CABALLUS)
    COZZI, B
    MOREI, G
    RAVAULT, JP
    CHESNEAU, D
    REITER, RJ
    [J]. JOURNAL OF PINEAL RESEARCH, 1991, 10 (03) : 130 - 135
  • [8] Denkova R, 2004, Endocr Regul, V38, P51
  • [9] The role of steroids in follicular growth
    Drummond, Ann E.
    [J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2006, 4 (1)
  • [10] EFFECT OF MELATONIN ALONE OR IN COMBINATION WITH HUMAN CHORIONIC-GONADOTROPIN OR OVINE LUTEINIZING-HORMONE ON THE INVITRO SECRETION OF ESTROGENS OR PROGESTERONE BY GRANULOSA-CELLS OF RATS
    FISKE, VM
    PARKER, KL
    ULMER, RA
    OW, CH
    AZIZ, N
    [J]. ENDOCRINOLOGY, 1984, 114 (02) : 407 - 410