Primer design for identifying economically important Liriomyza species (Diptera: Agromyzidae) by multiplex PCR

被引：27

作者：

Nakamura, Shigeo ^{[2
]}

Masuda, Toshio ^{[2
]}

Mochizuki, Atsushi ^{[1
]}

Konishi, Kazuhiko ^{[3
]}

Tokumaru, Susumu ^{[4
]}

Ueno, Keiichiro ^{[5
]}

Yamaguchi, Takuhiro ^{[5
]}

机构：

[1] Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan

[2] Miyagi Prefectural Inst Agr & Hort, Natori, Miyagi 9811243, Japan

[3] Natl Agr Res Ctr Hokkaido Reg, Toyohira Ku, Sapporo, Hokkaido 0628555, Japan

[4] Kyoto Prefectural Agr Forestry & Fisheries Techno, Uji, Kyoto 6110022, Japan

[5] Kagoshima Prefectural Inst Agr Dev, Kagoshima 8993401, Japan

来源：

MOLECULAR ECOLOGY RESOURCES | 2013年 / 13卷 / 01期

关键词：

cytochrome oxidase subunit I; invasive species; leafminer fly; mitochondria; multi-primers; HUIDOBRENSIS DIPTERA; TRIFOLII DIPTERA; MITOCHONDRIAL PHYLOGEOGRAPHY; SATIVAE DIPTERA; PEST; LEAFMINER; DIAGNOSIS; SUSCEPTIBILITY; BRYONIAE; CLADES;

D O I：

10.1111/1755-0998.12025

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Leafminer flies, especially, Liriomyza huidobrensis, Liriomyza sativae and Liriomyza trifolii, are quarantine species in many countries. Their morphological similarity makes identification difficult. To develop a rapid, reliable, sensitive and simple molecular identification method using multiplex PCR, we newly sequenced the mitochondrial cytochrome oxidase I (COI) genes of Liriomyza bryoniae, Liriomyza chinensis, L. huidobrensis, L. sativae, L. trifolii, Chromatomyia horticola and four parasitoid species. We aligned them with all the COI sequences of the leafminer flies found in the international DNA nucleotide sequence databases (DDBJ/EMBL/GenBank). We then designed species-specific primers to allow us to differentiate between L. bryoniae, L. chinensis, L. huidobrensis, L. sativae, and L. trifolii.

引用

页码：96 / 102

页数：7

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