Anti-tumor activity and the mechanism of SIP-S: A sulfated polysaccharide with anti-metastatic effect

被引：34

作者：

Zong, Aizhen ^{[1
,5
]}

Liu, Yuhong ^{[2
]}

Zhang, Yan ^{[1
]}

Song, Xinlei ^{[1
]}

Shi, Yikang ^{[3
]}

Cao, Hongzhi ^{[3
]}

Liu, Chunhui ^{[1
,3
]}

Cheng, Yanna ^{[4
]}

Jiang, Wenjie ^{[1
]}

Du, Fangling ^{[5
]}

Wang, Fengshan ^{[1
,3
]}

机构：

[1] Shandong Univ, Inst Biochem & Biotechnol Drugs, Sch Pharmaceut Sci, Key Lab Chem Biol,Minist Educ, Jinan 250012, Shandong, Peoples R China

[2] Shandong Univ Tradit Chinese Med, Sch Pharmaceut Sci, Jinan 250355, Shandong, Peoples R China

[3] Shandong Univ, Natl Glycoengn Res Ctr, Jinan 250012, Shandong, Peoples R China

[4] Shandong Univ, Sch Pharmaceut Sci, Dept Pharmacol, Jinan 250012, Shandong, Peoples R China

[5] Shandong Acad Agr Sci, Inst Agrofood Sci Technol, Jinan 250100, Shandong, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2015年 / 129卷

基金：

中国国家自然科学基金;

关键词：

SIP-S; Cyclophosphamide; Anti-tumor activity; Immunoenhancing activity; Apoptosis; SEPIELLA-MAINDRONI INK; CANCER; IMMUNOMODULATION; APOPTOSIS;

D O I：

10.1016/j.carbpol.2015.04.017

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Our previous studies demonstrated that SIP-S had anti-metastatic activity and inhibited the growth of metastatic foci. Here we report the anti-tumor and immunoregulatory potential of SIP-S. SIP-S could significantly inhibit tumor growth in S180-bearing mice, and the inhibition rates was 43.7% at 30 mg/kg d. Besides, SIP-S could improve the thymus and spleen indices of S180-bearing mice and the mice treated with CTX. The combination of SIP-S (15 mg/kg d) with CTX (12.5 mg/kg d) showed higher anti-tumor potency than CTX (25 mg/kg d) alone. These results indicated that SIP-S had immunoenhancing and anticancer activity, and the immunoenhancing activity might be one mechanism for its anti-tumor activity. Flow cytometry results showed that SIP-S could induce tumor cells apoptosis. Western blot analysis indicated that SIP-S could upregulate the expression of pro-apoptotic proteins, caspase-3, -8, -9 and Bax, and downregulate the expression of anti-apoptotic protein PARP-1 in tumor cells in a dose-dependent manner. In summary, SIP-S has anti-tumor activity, which may be associated with its immunostimulating and pro-apoptotic activity. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：50 / 54

页数：5

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