In situdetection of intracellular tissue transglutaminase based on aggregation-induced emission

被引：6
作者：
Wu, Yafeng [1 ]
Gao, Yaqiong [1 ]
Su, Juan [1 ]
Chen, Zixuan [1 ]
Liu, Songqin [1 ]
机构：
[1] Southeast Univ, Jiangsu Engn Lab Smart Carbon Rich Mat & Device, Sch Chem & Chem Engn, Nanjing 211189, Peoples R China
来源：
CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 63期
基金：
中国国家自然科学基金;
关键词：
CANCER CELLS; PROTEIN; QUANTIFICATION; APOPTOSIS; PROBE;
D O I：
10.1039/d0cc03365k
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Herein, a novel strategy forin situimaging and real-time monitoring of intracellular tissue transglutaminase (TG2) is presented based on aggregation-induced emission (AIE). It has high sensitivity and specificity, minimal background signal and can also effectively distinguish different cell types (drug-resistant cancer cells, cancer cells and normal cells).
引用
收藏
页码：9008 / 9011
页数：4
相关论文
共 31 条
  • [1] Adhikary Gautam, 2018, Oncotarget, V9, P34495, DOI 10.18632/oncotarget.26130
  • [2] Transglutaminase type 2 in the regulation of proteostasis
    D'Eletto, Manuela
    Rossin, Federica
    Fedorova, Olga
    Farrace, Maria Grazia
    Piacentini, Mauro
    [J]. BIOLOGICAL CHEMISTRY, 2019, 400 (02) : 125 - 140
  • [3] TGase-induced intracellular aggregation of Fe3O4 nanoparticles for increased retention and enhanced T2 MRI
    Gao, Xuefeng
    Liu, Yang
    Li, Yingying
    Niu, Xiaoyan
    Cao, Jing
    Li, Xiaomin
    Zhang, Yapei
    Tan, Dongzhi
    Wang, Wei
    Yuan, Zhi
    [J]. MATERIALS CHEMISTRY FRONTIERS, 2019, 3 (07) : 1365 - 1374
  • [4] Fluorometric assay for tissue transglutaminase-mediated transamidation activity
    Gnaccarini, Claudio
    Ben-Tahar, Wajih
    Lubell, William D.
    Pelletier, Joelle N.
    Keillor, Jeffrey W.
    [J]. BIOORGANIC & MEDICINAL CHEMISTRY, 2009, 17 (17) : 6354 - 6359
  • [5] Intracellular Localization and Conformational State of Transglutaminase 2: Implications for Cell Death
    Gundemir, Soner
    Johnson, Gail V. W.
    [J]. PLOS ONE, 2009, 4 (07):
  • [6] Guo Z., 2020, ANGEW CHEM INT EDIT, V59, P2
  • [7] Electrochemical Immunosensor Based on Nanoelectrode Ensembles for the Serological Analysis of IgG-type Tissue Transglutaminase
    Habtamu, Henok B.
    Not, Tarcisio
    De Leo, Luigina
    Longo, Sara
    Moretto, Ligia M.
    Ugo, Paolo
    [J]. SENSORS, 2019, 19 (05)
  • [8] Aggregation-induced emission
    Hong, Yuning
    Lam, Jacky W. Y.
    Tang, Ben Zhong
    [J]. CHEMICAL SOCIETY REVIEWS, 2011, 40 (11) : 5361 - 5388
  • [9] Multifunctional nanocatalyst-based ultrasensitive detection of human tissue transglutaminase 2
    Huang, Yue
    Li, Hao
    Fan, Qiongxuan
    Wang, Lei
    Wang, Yao
    Li, Genxi
    [J]. BIOSENSORS & BIOELECTRONICS, 2016, 83 : 85 - 90
  • [10] Crystal Structure of Transglutaminase 2 with GTP Complex and Amino Acid Sequence Evidence of Evolution of GTP Binding Site
    Jang, Tae-Ho
    Lee, Dong-Sup
    Choi, Kihang
    Jeong, Eui Man
    Kim, In-Gyu
    Kim, Young Whan
    Chun, Jung Nyeo
    Jeon, Ju-Hong
    Park, Hyun Ho
    [J]. PLOS ONE, 2014, 9 (09):
1234