Kinetics and Predicted Structure of a Novel Xylose Reductase from Chaetomium thermophilum

被引：10
作者：
Quehenberger, Julian [1 ]
Reichenbach, Tom [2 ]
Baumann, Niklas [1 ]
Rettenbacher, Lukas [1 ]
Divne, Christina [2 ]
Spadiut, Oliver [1 ]
机构：
[1] TU Wien, Fac Tech Chem, Inst Chem Environm & Biosci Engn, Res Div Biochem Engn, A-1060 Vienna, Austria
[2] KTH Sch Engn Sci Chem Biotechnol & Hlth, SE-10044 Stockholm, Sweden
来源：
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 01期
关键词：
xylose reductase; Chaetomium thermophilum; kinetics; structure; homology model; cofactor binding; stability; ALDO-KETO REDUCTASE; COENZYME SPECIFICITY; HETEROLOGOUS EXPRESSION; ETHANOL-PRODUCTION; CANDIDA-TENUIS; CLONING; XYLITOL; PURIFICATION; CATALYSIS; COMPLEX;
D O I：
10.3390/ijms20010185
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
While in search of an enzyme for the conversion of xylose to xylitol at elevated temperatures, a xylose reductase (XR) gene was identified in the genome of the thermophilic fungus Chaetomium thermophilum. The gene was heterologously expressed in Escherichia coli as a His6-tagged fusion protein and characterized for function and structure. The enzyme exhibits dual cofactor specificity for NADPH and NADH and prefers D-xylose over other pentoses and investigated hexoses. A homology model based on a XR from Candida tenuis was generated and the architecture of the cofactor binding site was investigated in detail. Despite the outstanding thermophilicity of its host the enzyme is, however, not thermostable.
引用
收藏
页数：17
相关论文
共 36 条
  • [1] Insight into Structure and Assembly of the Nuclear Pore Complex by Utilizing the Genome of a Eukaryotic Thermophile
    Amlacher, Stefan
    Sarges, Phillip
    Flemming, Dirk
    van Noort, Vera
    Kunze, Ruth
    Devos, Damien P.
    Arumugam, Manimozhiyan
    Bork, Peer
    Hurt, Ed
    [J]. CELL, 2011, 146 (02) : 277 - 289
  • [2] Xylose reductase from Pichia stipitis with altered coenzyme preference improves ethanolic xylose fermentation by recombinant Saccharomyces cerevisiae
    Bengtsson, Oskar
    Hahn-Hagerdal, Barbel
    Gorwa-Grauslund, Marie F.
    [J]. BIOTECHNOLOGY FOR BIOFUELS, 2009, 2
  • [3] An integrated approach for genome annotation of the eukaryotic thermophile Chaetomium thermophilum
    Bock, Thomas
    Chen, Wei-Hua
    Ori, Alessandro
    Malik, Nayab
    Silva-Martin, Noella
    Huerta-Cepas, Jaime
    Powell, Sean T.
    Kastritis, Panagiotis L.
    Smyshlyaev, Georgy
    Vonkova, Ivana
    Kirkpatrick, Joanna
    Doerks, Tobias
    Nesme, Leo
    Bassler, Jochen
    Kos, Martin
    Hurt, Ed
    Carlomagno, Teresa
    Gavin, Anne-Claude
    Barabas, Orsolya
    Mueller, Christoph W.
    van Noort, Vera
    Beck, Martin
    Bork, Peer
    [J]. NUCLEIC ACIDS RESEARCH, 2014, 42 (22) : 13525 - 13533
  • [4] Challenges and prospects of xylitol production with whole cell bio-catalysis: A review
    Dasgupta, Diptarka
    Bandhu, Sheetal
    Adhikari, Dilip K.
    Ghosh, Debashish
    [J]. MICROBIOLOGICAL RESEARCH, 2017, 197 : 9 - 21
  • [5] Biotechnological production of xylitol from lignocellulosic wastes: A review
    de Albuquerque, Tiago Lima
    da Silva, Ivanildo Jose, Jr.
    de Macedo, Gorete Ribeiro
    Ponte Rocha, Maria Valderez
    [J]. PROCESS BIOCHEMISTRY, 2014, 49 (11) : 1779 - 1789
  • [6] DeLisa MP, 1999, BIOTECHNOL BIOENG, V65, P54, DOI 10.1002/(SICI)1097-0290(19991005)65:1<54::AID-BIT7>3.3.CO
  • [7] 2-I
  • [8] Comparisons of Six New Artificial Sweetener Gradation Ratios with Sucrose in Conventional-Method Cupcakes Resulting in Best Percentage Substitution Ratios
    Edelstein, Sari
    Smith, Khalia
    Worthington, Adriene
    Gillis, Nicole
    Bruen, Diana
    Kang, Sun Hyung
    Ho, Wai Ling
    Gilpin, Kathy
    Ackerman, Jennifer
    Guiducci, Gina
    [J]. JOURNAL OF CULINARY SCIENCE & TECHNOLOGY, 2007, 5 (04) : 61 - 74
  • [9] Features and development of Coot
    Emsley, P.
    Lohkamp, B.
    Scott, W. G.
    Cowtan, K.
    [J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 2010, 66 : 486 - 501
  • [10] Xylose reductase from the thermophilic fungus Talaromyces emersonii: cloning and heterologous expression of the native gene (Texr) and a double mutant (Texr K271R+N273D) with altered coenzyme specificity
    Fernandes, Sara
    Tuohy, Maria G.
    Murray, Patrick G.
    [J]. JOURNAL OF BIOSCIENCES, 2009, 34 (06) : 881 - 890
1234