Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation

被引：41
作者：
Ait-Belkacem, Dora [1 ]
Guilbert, Marie [2 ]
Roche, Muriel [1 ]
Duboisset, Julien [1 ]
Ferrand, Patrick [1 ]
Sockalingum, Ganesh [2 ]
Jeannesson, Pierre [2 ]
Brasselet, Sophie [1 ]
机构：
[1] Aix Marseille Univ, Inst Fresnel, Ecole Cent, CNRS,UMR 7249, F-13013 Marseille, France
[2] Univ Reims, MeDIAN, FRE CNRS URCA 3481, UFR Pharm, F-51096 Reims, France
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2012年 / 17卷 / 08期
关键词：
aging; collagen fibrils; microscopy; molecular order; polarization; second-harmonic generation; GLYCATION;
D O I：
10.1117/1.JBO.17.8.080506
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Polarization resolved second harmonic generation (PSHG) is developed to study, at the microscopic scale, the impact of aging on the structure of type I collagen fibrils in two-dimensional coatings. A ribose-glycated collagen is also used to mimic tissue glycation usually described as an indicator of aging. PSHG images are analyzed using a generic approach of the molecular disorder information in collagen fibrils, revealing significant changes upon aging, with a direct correlation between molecular disorder and fibril diameters. (C) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.8.080506]
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页数：3
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