Crude Oil Impairs Cardiac Excitation-Contraction Coupling in Fish

被引：230

作者：

Brette, Fabien ^{[1
]}

Machado, Ben ^{[1
]}

Cros, Caroline ^{[1
]}

Incardona, John P. ^{[2
]}

Scholz, Nathaniel L. ^{[2
]}

Block, Barbara A. ^{[1
]}

机构：

[1] Stanford Univ, Hopkins Marine Stn, Dept Biol, Pacific Grove, CA 93950 USA

[2] NOAA, NW Fisheries Sci Ctr, Seattle, WA 98112 USA

来源：

SCIENCE | 2014年 / 343卷 / 6172期

关键词：

POLYCYCLIC AROMATIC-HYDROCARBONS; HERRING CLUPEA-PALLASI; ATLANTIC BLUEFIN TUNA; VENTRICULAR MYOCYTES; EMBRYOS; MORTALITY; EXPOSURE; SENSITIVITY; PLASTICITY; ZEBRAFISH;

D O I：

10.1126/science.1242747

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Crude oil is known to disrupt cardiac function in fish embryos. Large oil spills, such as the Deepwater Horizon (DWH) disaster that occurred in 2010 in the Gulf of Mexico, could severely affect fish at impacted spawning sites. The physiological mechanisms underlying such potential cardiotoxic effects remain unclear. Here, we show that crude oil samples collected from the DWH spill prolonged the action potential of isolated cardiomyocytes from juvenile bluefin and yellowfin tunas, through the blocking of the delayed rectifier potassium current (I-Kr). Crude oil exposure also decreased calcium current (I-Ca) and calcium cycling, which disrupted excitation-contraction coupling in cardiomyocytes. Our findings demonstrate a cardiotoxic mechanism by which crude oil affects the regulation of cellular excitability, with implications for life-threatening arrhythmias in vertebrates.

引用

页码：772 / 776

页数：5

共 32 条

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