The epigenetically-regulated miR-663 targets H-ras in K-562 cells

被引：20

作者：

Yang, Yang ^{[1
,2
,3
]}

Wang, Li-Li ^{[1
,2
]}

Wang, Heng-Xiang ^{[3
]}

Guo, Zi-Kuan ^{[4
]}

Gao, Xiao-Fang ^{[5
]}

Cen, Jian ^{[6
]}

Li, Yong-Hui ^{[1
,2
]}

Dou, Li-Ping ^{[1
,2
]}

Yu, Li ^{[1
,2
]}

机构：

[1] Chinese Peoples Liberat Army Gen Hosp, Dept Haematol, Beijing, Peoples R China

[2] Chinese Peoples Liberat Army Gen Hosp, BMT Ctr, Beijing, Peoples R China

[3] Chinese PLA AF Gen Hosp, Dept Haematol, Beijing, Peoples R China

[4] Beijing Inst Radiat Med, Beijing, Peoples R China

[5] Chinese Peoples Liberat Army Gen Hosp, Dept Respirat, Beijing, Peoples R China

[6] Chinese PLA Navy Gen Hosp, Dept Haematol, Beijing, Peoples R China

来源：

FEBS JOURNAL | 2013年 / 280卷 / 20期

基金：

中国国家自然科学基金;

关键词：

DNA methylation; epigenetics; gene expression; H-ras; MiR-663; TUMOR-SUPPRESSOR; MICRORNAS; CANCER; GENE; KRAS; TUMORIGENESIS; METHYLATION; EXPRESSION; PATHWAY;

D O I：

10.1111/febs.12485

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

miR-663 is a tumour suppressor that is potentially regulated by modification of CpG islands. Whether aberrant methylation is one of the reasons for miR-663 down-regulation in some malignant cells and whether miR-663 targets oncogenes warrants further research. In the present study, we report that the CpG islands in the upstream region of pre-miR-663 are aberrantly methylated in the k-562 cell line and in the white blood cells of some chronic myelogenous leukaemia patients, and also that H-ras is one of the genes targeted by miR-663. Over-expression of miR-663 may suppress proliferation of the k-562 cell line in part by enhancing cell apoptosis.

引用

页码：5109 / 5117

页数：9

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