Gut microbiota composition and bone mineral lossepidemiologic evidence from individuals in Wuhan, China

被引:150
作者
Li, C. [1 ]
Huang, Q. [2 ]
Yang, R. [3 ]
Dai, Y. [4 ]
Zeng, Y. [5 ]
Tao, L. [1 ]
Li, X. [1 ]
Zeng, J. [3 ]
Wang, Q. [1 ]
机构
[1] Huazhong Univ Sci & Technol, MOE Key Lab Environm & Hlth, Dept Epidemiol & Biostat, Sch Publ Hlth,Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
[2] Huazhong Univ Sci & Technol, Dept Rehabil Med, Union Hosp, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
[3] Huazhong Univ Sci & Technol, Dept Hlth Checkup, Union Hosp, Tongji Med Coll, Wuhan 430030, Hubei, Peoples R China
[4] Huazhong Univ Sci & Technol, Tongji Med Coll, Dept Nucl Med, Union Hosp, Wuhan 430030, Hubei, Peoples R China
[5] Wuhan 1 Hosp, Wuhan 430030, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
Bone mineral loss; Gut microbiota; Osteoporosis; 16S Ribosomal RNA; Sequencing; ACUTE-INFLAMMATION; HEALTH; OSTEOPOROSIS; METABOLITES; MASS; HYPERTENSION; OSTEOBLAST; ENDOTOXIN; BUTYRATE; BACTERIA;
D O I
10.1007/s00198-019-04855-5
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The SummaryWe explored the association between gut microbiota composition and bone mineral loss in Chinese elderly people by high-throughput 16S ribosomal RNA (rRNA) gene sequencing. Compared with controls, a smaller number of operational taxonomic units (OTUs), several taxa with altered abundance, and specific functional pathways were found in individuals with low-bone mineral density (BMD).IntroductionGut microbiota plays important roles in human health and associates with a number of diseases. However, few studies explored its association with bone mineral loss in human.MethodsWe collected 102 fecal samples from each eligible individual belonging to low-BMD and control groups for high-throughput 16S rRNA gene sequencing.ResultsThe low-BMD individuals had a smaller number of OTUs and bacterial taxa at each level. At the phylum level, Bacteroidetes were more abundant in the low-BMD group; Firmicutes were enriched in the control group; Firmicutes and Actinobacteria positively correlated and Bacteroidetes negatively correlated with the BMD and T-score in all subjects. At the family level, the abundance of Lachnospiraceae in low-BMD individuals reduced and positively correlated with BMD and T-score; meanwhile, BMD increased with increasing Bifidobacteriaceae. At the genus level, low-BMD individuals had decreased proportions of Roseburia compared with control ones (P<0.05). Roseburia, Bifidobacterium, and Lactobacillus positively correlated with BMD and T-score. Furthermore, BMD increased with rising abundance of Bifidobacterium. Functional prediction revealed that 93 metabolic pathways significantly differed between the two groups (FDR-corrected P<0.05). Most pathways, especially pathways related to LPS biosynthesis, were more abundant in low-BMD individuals than in control ones.ConclusionsSeveral taxa with altered abundance and specific functional pathways were discovered in low-BMD individuals. Our findings provide novel epidemiologic evidence to elucidate the underlying microbiota-relevant mechanism in bone mineral loss and osteoporosis.
引用
收藏
页码:1003 / 1013
页数:11
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