Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with In Vitro Lytic Activity against Bovine MastitisStreptococcus uberis

被引:33
作者
Vander Elst, Niels [1 ,2 ,3 ,4 ]
Linden, Sara B. [2 ]
Lavigne, Rob [3 ]
Meyer, Evelyne [1 ]
Briers, Yves [4 ]
Nelson, Daniel C. [2 ]
机构
[1] Univ Ghent, Fac Vet Med, Dept Pharmacol Toxicol & Biochem, Lab Biochem, B-9820 Merelbeke, Belgium
[2] Univ Maryland, Inst Biosci & Biotechnol Res, Lab Antimicrobial Discovery, Rockville, MD 20850 USA
[3] Katholieke Univ Leuven, Dept Biosyst, Lab Gene Technol, B-3001 Heverlee, Belgium
[4] Univ Ghent, Dept Biotechnol, Lab Appl Biotechnol, B-9000 Ghent, Belgium
来源
ANTIBIOTICS-BASEL | 2020年 / 9卷 / 09期
关键词
PlySs2; PlySs9; bacteriophage-derived endolysins; bovine mastitis; dairy industry; intramammary antibiotics; alternative antimicrobials; STAPHYLOCOCCUS-AUREUS; MASTITIS PATHOGENS; DAIRY-COWS; ANTIBACTERIAL ACTIVITY; STREPTOCOCCUS-UBERIS; CLINICAL MASTITIS; PHAGE LYSIN; MOUSE MODEL; INFECTION;
D O I
10.3390/antibiotics9090621
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Bovine mastitis, an infection of the cow's mammary gland, is frequently caused byStreptococcus uberisand causes major economic losses in the dairy industry. The intramammary administration of antibiotics currently remains the predominant preventive and therapeutic measure. These antimicrobial compounds, of which some are considered critical in human health care, are frequently applied as dry therapy resulting in their consistent overuse. Therefore, the use of antibiotics in the dairy sector is being questioned. We here identified two endolysins, i.e., PlySs2 and PlySs9, respectively derived fromStreptococcus suisserotype-2 and -9 prophages, with lytic activity againstS. uberisin an in vitro setting. Both endolysins gave clear lysis zones in spot-on-plate assays and caused a reduction of the optical density in a turbidity reduction assay. In depth characterization identified PlySs9 as the more potent endolysin over PlySs2 with a lower MIC value and about one additional log of killing. PlySs2 and PlySs9 were challenged to a panel of subclinical and clinicalS. uberismilk isolates and were both able to lyse all strains tested. Molecular dissection of these endolysins in catalytic and cell wall binding subdomains resulted in major loss of killing and binding activity, respectively. Taken together, we here propose PlySs2 and PlySs9 as candidate compounds to the current antimicrobial arsenal known against bovine mastitis-causingS. uberisas future add-on or replacement strategy to the currently used intramammary antibiotics.
引用
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页码:1 / 14
页数:14
相关论文
共 44 条
[41]   Antimicrobial Activity of Exebacase (Lysin CF-301) against the Most Common Causes of Infective Endocarditis [J].
Watson, Aubrey ;
Oh, Jun Taek ;
Sauve, Karen ;
Bradford, Patricia A. ;
Cassino, Cara ;
Schuch, Raymond .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2019, 63 (10)
[42]  
World Health Organization (WHO), 2018, CRIT IMP ANT HUM MED
[43]   Bacteriophages and associated endolysins in therapy and prevention of mastitis and metritis in cows: Current knowledge [J].
Zdunczyk, Slawomir ;
Janowski, Tomasz .
ANIMAL REPRODUCTION SCIENCE, 2020, 218
[44]   PREVENTIVE METHODS IN REDUCTION OF MASTITIS PATHOGENS IN DAIRY COWS [J].
Zigo, Frantisek ;
Elecko, Juraj ;
Farkasova, Zuzana ;
Zigova, Martina ;
Vasil, Milan ;
Ondrasovicova, Silvia ;
Lenka, Kudelkova .
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES, 2019, 9 (01) :121-126