Characterization of the Bacteriophage-Derived Endolysins PlySs2 and PlySs9 with In Vitro Lytic Activity against Bovine MastitisStreptococcus uberis

被引:28
作者
Vander Elst, Niels [1 ,2 ,3 ,4 ]
Linden, Sara B. [2 ]
Lavigne, Rob [3 ]
Meyer, Evelyne [1 ]
Briers, Yves [4 ]
Nelson, Daniel C. [2 ]
机构
[1] Univ Ghent, Fac Vet Med, Dept Pharmacol Toxicol & Biochem, Lab Biochem, B-9820 Merelbeke, Belgium
[2] Univ Maryland, Inst Biosci & Biotechnol Res, Lab Antimicrobial Discovery, Rockville, MD 20850 USA
[3] Katholieke Univ Leuven, Dept Biosyst, Lab Gene Technol, B-3001 Heverlee, Belgium
[4] Univ Ghent, Dept Biotechnol, Lab Appl Biotechnol, B-9000 Ghent, Belgium
来源
ANTIBIOTICS-BASEL | 2020年 / 9卷 / 09期
关键词
PlySs2; PlySs9; bacteriophage-derived endolysins; bovine mastitis; dairy industry; intramammary antibiotics; alternative antimicrobials; STAPHYLOCOCCUS-AUREUS; MASTITIS PATHOGENS; DAIRY-COWS; ANTIBACTERIAL ACTIVITY; STREPTOCOCCUS-UBERIS; CLINICAL MASTITIS; PHAGE LYSIN; MOUSE MODEL; INFECTION;
D O I
10.3390/antibiotics9090621
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Bovine mastitis, an infection of the cow's mammary gland, is frequently caused byStreptococcus uberisand causes major economic losses in the dairy industry. The intramammary administration of antibiotics currently remains the predominant preventive and therapeutic measure. These antimicrobial compounds, of which some are considered critical in human health care, are frequently applied as dry therapy resulting in their consistent overuse. Therefore, the use of antibiotics in the dairy sector is being questioned. We here identified two endolysins, i.e., PlySs2 and PlySs9, respectively derived fromStreptococcus suisserotype-2 and -9 prophages, with lytic activity againstS. uberisin an in vitro setting. Both endolysins gave clear lysis zones in spot-on-plate assays and caused a reduction of the optical density in a turbidity reduction assay. In depth characterization identified PlySs9 as the more potent endolysin over PlySs2 with a lower MIC value and about one additional log of killing. PlySs2 and PlySs9 were challenged to a panel of subclinical and clinicalS. uberismilk isolates and were both able to lyse all strains tested. Molecular dissection of these endolysins in catalytic and cell wall binding subdomains resulted in major loss of killing and binding activity, respectively. Taken together, we here propose PlySs2 and PlySs9 as candidate compounds to the current antimicrobial arsenal known against bovine mastitis-causingS. uberisas future add-on or replacement strategy to the currently used intramammary antibiotics.
引用
收藏
页码:1 / 14
页数:14
相关论文
共 44 条
[31]   In Vitro Characterization of PlySK1249, a Novel Phage Lysin, and Assessment of Its Antibacterial Activity in a Mouse Model of Streptococcus agalactiae Bacteremia [J].
Oechslin, Frank ;
Daraspe, Jean ;
Giddey, Marlyse ;
Moreillon, Philippe ;
Resch, Gregory .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (12) :6276-6283
[32]   Bacteriophage-encoded depolymerases: their diversity and biotechnological applications [J].
Pires, Diana P. ;
Oliveira, Hugo ;
Melo, Luis D. R. ;
Sillankorva, Sanna ;
Azeredo, Joana .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 100 (05) :2141-2151
[33]   Vaccination of dairy cows with recombinant Streptococcus uberis adhesion molecule induces antibodies that reduce adherence to and internalization of S. uberis into bovine mammary epithelial cells [J].
Prado, M. E. ;
Almeida, R. A. ;
Ozen, C. ;
Luther, D. A. ;
Lewis, M. J. ;
Headrick, S. J. ;
Oliver, S. P. .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2011, 141 (3-4) :201-208
[34]  
Radzikowski D, 2020, ANIM SCI PAP REP, V38, P117
[35]   Synergistic streptococcal phage λSA2 and B30 endolysins kill streptococci in cow milk and in a mouse model of mastitis [J].
Schmelcher, Mathias ;
Powell, Anne M. ;
Camp, Mary J. ;
Pohl, Calvin S. ;
Donovan, David M. .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2015, 99 (20) :8475-8486
[36]   Evolutionarily distinct bacteriophage endolysins featuring conserved peptidoglycan cleavage sites protect mice from MRSA infection [J].
Schmelcher, Mathias ;
Shen, Yang ;
Nelson, Daniel C. ;
Eugster, Marcel R. ;
Eichenseher, Fritz ;
Hanke, Daniela C. ;
Loessner, Martin J. ;
Dong, Shengli ;
Pritchard, David G. ;
Lee, Jean C. ;
Becker, Stephen C. ;
Foster-Frey, Juli ;
Donovan, David M. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2015, 70 (05) :1453-1465
[37]   Comparative genomic analysis of twelve Streptococcus suis (pro)phages [J].
Tang, Fang ;
Bossers, Alex ;
Harders, Frank ;
Lu, Chengping ;
Smith, Hilde .
GENOMICS, 2013, 101 (06) :336-344
[38]   Factors associated with intramammary infection in dairy cows caused by coagulase-negative staphylococci, Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, Corynebacterium bovis, or Escherichia coil [J].
Taponen, S. ;
Liski, E. ;
Heikkila, A. -M. ;
Pyorala, S. .
JOURNAL OF DAIRY SCIENCE, 2017, 100 (01) :493-503
[39]  
Totté JEE, 2017, CASE REP DERMATOL, V9, P19, DOI 10.1159/000473872
[40]   Potential therapeutic application of bacteriophages and phage-derived endolysins as alternative treatment of bovine mastitis [J].
Vander Elst, N. ;
Meyer, E. .
VLAAMS DIERGENEESKUNDIG TIJDSCHRIFT, 2018, 87 (04) :181-187