Semiconductor nanocrystal probes for human metaphase chromosomes

被引：142

作者：

Xiao, Y ^{[1
]}

Barker, PE ^{[1
]}

机构：

[1] NIST, NCI, Biomarkers Validat Lab, DNA Technol Grp,Chem Sci & Technol Lab, Gaithersburg, MD 20899 USA

来源：

NUCLEIC ACIDS RESEARCH | 2004年 / 32卷 / 03期

关键词：

D O I：

10.1093/nar/gnh024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photostable than organic fluorophores Texas Red and fluorescein. Thus, semiconductor nanocrystal fluorophores offer a more stable and quantitative mode of fluorescence in situ hybridization (FISH) for research and clinical applications.

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页数：9

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