Critical role of leukotriene B4 receptor signaling in mouse 3T3-L1 preadipocyte differentiation

被引：8

作者：

Hirata, Kae ^{[1
,2
]}

Wada, Koichiro ^{[2
]}

Murata, Yuka ^{[1
,2
]}

Nakajima, Atsushi ^{[3
]}

Yamashiro, Takashi ^{[1
]}

Kamisaki, Yoshinori ^{[2
]}

机构：

[1] Osaka Univ, Grad Sch Dent, Dept Orthodont & Dentofacial Orthoped, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Grad Sch Dent, Dept Pharmacol, Suita, Osaka 5650871, Japan

[3] Yokohama City Univ, Sch Med, Div Gastroenterol, Yokohama, Kanagawa 2360004, Japan

来源：

LIPIDS IN HEALTH AND DISEASE | 2013年 / 12卷

基金：

日本学术振兴会;

关键词：

Leukotrienes; Preadipocyte differentiation; Mouse; 3T3-L1; fibroblasts; BLT; siRNA; STEM-CELL PROLIFERATION; INSULIN-RESISTANCE; LIPOXIN; GAMMA; LINE; A(4);

D O I：

10.1186/1476-511X-12-122

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Various inflammatory mediators related to obesity might be closely related to insulin resistance. Leukotrienes (LTs) are involved in inflammatory reactions. However, there are few reports regarding the role of LTs in adipocyte differentiation. Therefore, we investigated the role of leukotriene B-4 (LTB4)-leukotriene receptor (BLT) signaling in mouse 3T3-L1 fibroblastic preadipocyte differentiation to mature adipocytes. Methods: Mouse 3T3-L1 preadipocytes were treated with lipoxygenase (LOX) inhibitors, BLT antagonist, and small interfering RNA (siRNA) for BLT1 and BLT2 to block the LTB4-BLT signaling pathway, then the adipocyte differentiation such as lipid accumulation and the increase in triglyceride was evaluated. Results: Blockade of BLT signaling by treatment with a LOX inhibitor or a BLT antagonist suppressed preadipocyte differentiation into mature adipocytes. In addition, knockdown of BLT1 and BLT2 by siRNAs dramatically inhibited differentiation. These results indicate the LTB4-BLT signaling pathway may positively regulate preadipocyte differentiation and be a rate-limiting system to control adipocyte differentiation. Conclusions: The LTB4-BLT signaling pathway provides a potent regulatory signal that accelerates the differentiation of mouse 3T3-L1 preadipocytes. Further investigations are necessary to confirm the exact role of LTB4 and BLTs signaling pathways in preadipocyte differentiation.

引用

页数：7

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