Singlet oxygen-induced DNA damage

被引：38

作者：

DeFedericis, HC ^{[1
]}

Patrzyc, HB ^{[1
]}

Rajecki, MJ ^{[1
]}

Budzinski, EE ^{[1
]}

Iijima, H ^{[1
]}

Dawidzik, JB ^{[1
]}

Evans, MS ^{[1
]}

Greene, KF ^{[1
]}

Box, HC ^{[1
]}

机构：

[1] Roswell Pk Canc Inst, Dept Cellular Stress Biol, Buffalo, NY 14263 USA

来源：

RADIATION RESEARCH | 2006年 / 165卷 / 04期

关键词：

D O I：

10.1667/RR3533.1

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Singlet oxygen, hydrogen peroxide, hydroxyl radical and hydrogen peroxide are the reactive oxygen species (ROS) considered most responsible for producing oxidative stress in cells and organisms. Singlet oxygen interacts preferentially with guanine to produce 8-oxo-7,8-dihydroguanine and spiroiminodihydantoin. DNA damage due to the latter lesion has not been detected directly in the DNA of cells exposed to singlet oxygen. In this study, the singlet oxygen-induced lesion was isolated from a short synthetic oligomer after exposure to UVA radiation in the presence of methylene blue. The lesion could be enzymatically excised from the oligomer in the form of a modified dinucleoside monophosphate. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), the singlet oxygen lesion was detected in the form of modified dinucleoside monophosphates in double-stranded DNA and in the DNA of HeLa cells exposed to singlet oxygen. Pentamer containing the singlet oxygen-induced lesion and an isotopic label was synthesized as an internal standard for quantifying the lesion and served as well as for correcting for losses of product during sample preparation. (c) 2006 by Radiation Research Society.

引用

页码：445 / 451

页数：7

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