Investigation into allergenicity reduction and glycation sites of glycated β-lactoglobulin with ultrasound pretreatment by high-resolution mass spectrometry

被引：76
作者：
Liu, Guang-xian [1 ,3 ]
Tu, Zong-cai [1 ,2 ]
Yang, Wenhua [2 ]
Wang, Hui [2 ]
Zhang, Lu [1 ]
Ma, Da [2 ]
Huang, Tao [2 ]
Liu, Jun [2 ]
Li, Xue [2 ]
机构：
[1] Jiangxi Normal Univ, Minist Educ, Key Lab Funct Small Organ Mol, Nanchang 330022, Jiangxi, Peoples R China
[2] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China
[3] Jiangxi Acad Agr Sci, Nanchang 330200, Jiangxi, Peoples R China
来源：
FOOD CHEMISTRY | 2018年 / 252卷
基金：
中国国家自然科学基金;
关键词：
beta-Lactoglobulin; Allergenicity; Specific glycation sites; Antigenic epitopes; Ultrasound pretreatment; Glycation; HIGH-INTENSITY-ULTRASOUND; LIQUID-CHROMATOGRAPHY; MAILLARD REACTION; CONFORMATIONAL-CHANGES; STRUCTURAL-CHANGES; ANTIGENICITY; OVALBUMIN; PROTEINS; DEXTRAN; IGE;
D O I：
10.1016/j.foodchem.2018.01.086
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Ultrasound treatment could change the conformation of beta-lactoglobulin (beta-Lg) and improve the glycation reaction in aqueous solution under neutral condition. However, the effect of ultrasound pretreatment on glycation of beta-Lg with pentose at dry-state remains ambiguous, and the relationship between glycation and allergenicity of beta-Lg with ultrasound pretreatment is unclear. This study aimed to evaluate the effect of ultrasound pretreatment on glycation and allergenicity of beta-Lg. Markedly decreased allergenicity of beta-Lg was observed after glycation with ribose before and after ultrasound pretreatment with the minimum found at 400 W. Orbitrap LC-MS/ MS showed that the glycation degree of some peptides in glycated beta-Lg with and without ultrasound pretreatment were different although the content of free amino group and molecular mass were insignificantly different. Therefore, ultrasound pretreatment promoted the reduction in allergenicity by improving the glycation extent of some glycation sites although it hardly enhanced the whole glycation degree of beta-Lg.
引用
收藏
页码：99 / 107
页数：9
相关论文
共 50 条
  • [31] β-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry
    Capitan, Florina
    Robu, Adrian C.
    Schiopu, Catalin
    Ilie, Constantin
    Chait, Brian T.
    Przybylski, Michael
    Zamfir, Alina D.
    [J]. AMINO ACIDS, 2015, 47 (11) : 2399 - 2407
  • [32] Comparison of ovalbumin glycation induced by high-temperature steaming and high-temperature baking: A study combining conventional spectroscopy with high-resolution mass spectrometry
    Hu, Yueming
    Chen, Haiqi
    Yang, Yifan
    Zhang, Siqiong
    Xie, Zuohua
    Liu, Jiaojiao
    Jiang, Qiannan
    Liu, Jun
    Wen, Pingwei
    [J]. FOOD RESEARCH INTERNATIONAL, 2023, 173
  • [33] Capture modes evaluation of four flavor constituents in β-lactoglobulin by high-resolution mass spectrometry and molecular dynamics simulation approaches
    Di, Chenna
    Jia, Wei
    [J]. FOOD HYDROCOLLOIDS, 2023, 145
  • [34] High-resolution mass spectrometry: Theoretical and technological aspects
    Zarrouk, Elies
    Lenski, Marie
    Bruno, Clement
    Thibert, Valerie
    Contreras, Paul
    Privat, Kevin
    Ameline, Alice
    Fabresse, Nicolas
    [J]. TOXICOLOGIE ANALYTIQUE ET CLINIQUE, 2022, 34 (01) : 3 - 18
  • [35] YEAST EXPRESSION PROTEOMICS BY HIGH-RESOLUTION MASS SPECTROMETRY
    Walther, Tobias C.
    Olsen, Jesper V.
    Mann, Matthias
    [J]. METHODS IN ENZYMOLOGY, VOL 470: GUIDE TO YEAST GENETICS:: FUNCTIONAL GENOMICS, PROTEOMICS, AND OTHER SYSTEMS ANALYSIS, 2ND EDITION, 2010, 470 : 259 - 280
  • [36] Ozone-induced damage of fibrinogen molecules: identification of oxidation sites by high-resolution mass spectrometry
    Yurina, Lyubov
    Vasilyeva, Alexandra
    Indeykina, Maria
    Bugrova, Anna
    Biryukova, Marina
    Kononikhin, Alexey
    Nikolaev, Evgene
    Rosenfeld, Mark
    [J]. FREE RADICAL RESEARCH, 2019, 53 (04) : 430 - 455
  • [37] Investigation of Biotransformation Products of p-Methoxymethylamphetamine and Dihydromephedrone in Wastewater by High-Resolution Mass Spectrometry
    Kinyua, Juliet
    Psoma, Aikaterini K.
    Rousis, Nikolaos I.
    Nika, Maria-Christina
    Covaci, Adrian
    van Nuijs, Alexander L. N.
    Thomaidis, Nikolaos S.
    [J]. METABOLITES, 2021, 11 (02) : 1 - 12
  • [38] Reference Standardization for Mass Spectrometry and High-resolution Metabolomics Applications to Exposome Research
    Go, Young-Mi
    Walker, Douglas I.
    Liang, Yongliang
    Uppal, Karan
    Soltow, Quinlyn A.
    ViLinh Tran
    Strobel, Frederick
    Quyyumi, Arshed A.
    Ziegler, Thomas R.
    Pennell, Kurt D.
    Miller, Gary W.
    Jones, Dean P.
    [J]. TOXICOLOGICAL SCIENCES, 2015, 148 (02) : 531 - 543
  • [39] A model study on the site-specificity of (-)-epicatechin-induced reactions in β-lactoglobulin by high-resolution mass spectrometry in combination with bioinformatics
    Boersig, Amelie
    Konar, Nevzat
    Dalabasmaz, Sevim
    [J]. FOOD CHEMISTRY, 2023, 408
  • [40] Increase of Ovalbumin Glycation by the Maillard Reaction after Disruption of the Disulfide Bridge Evaluated by Liquid Chromatography and High Resolution Mass Spectrometry
    Huang, Xiaoqin
    Tu, Zongcai
    Wang, Hui
    Zhang, Qiuting
    Shi, Yan
    Xiao, Hui
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 61 (09) : 2253 - 2262
12345