Proteolytic and amylolytic enzymes from a newly isolated Bacillus mojavensis SA: Characterization and applications as laundry detergent additive and in leather processing

被引：58
作者：
Hammami, Amal [1 ]
Fakhfakh, Nahed [1 ]
Abdelhedi, Ola [1 ]
Nasri, Moncef [1 ]
Bayoudh, Ahmed [1 ]
机构：
[1] Univ Sfax, Engn Natl Sch Sfax ENIS, Lab Enzyme Engn & Microbiol, Sfax, Tunisia
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 108卷
关键词：
Bacillus mojavensis; Alkaline proteases; alpha-Amylases; Detergent stable; Dehairing function; THERMOSTABLE ALKALINE PROTEASE; ALPHA-AMYLASE; SHRIMP WASTES; PURIFICATION; STRAIN; OPTIMIZATION; EXTRACTION; CLEAVAGE; SEAWATER; TOLERANT;
D O I：
10.1016/j.ijbiomac.2017.11.148
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The present work aims to study the simultaneous production of highly alkaline proteases and thermostable alpha-amylases by a newly isolated bacterium Bacillus mojavensis SA. The optimum pH and temperature of amylase activity were 9.0 and 55 degrees C, respectively, while those of the proteolytic activity were 12.0 and 60 degrees C, respectively. Both alpha-amylase and protease enzymes showed a high stability towards a wide range of pH and temperature. Furthermore, SA crude enzymes were relatively stable towards non-ionic (Tween 20, Tween 80 and Triton X-100) and anionic (SDS) surfactants, as well as oxidizing agents. Both activities were improved by the presence of polyethylene glycol 4000 and glycerol. Additionally, the crude enzymes showed excellent stability against various solid and liquid detergents. Wash performance analysis revealed that the SA crude enzymes exhibited a remarkable efficiency in the removal of a variety type of stains, such as blood, chocolate, coffee and oil. On the other side, SA proteases revealed a potential dehairing activity of animal hide without chemical assistance or fibrous proteins hydrolysis. Thus, considering their promising properties, B. mojavensis SA crude enzymes could be used in several biotechnological bioprocesses. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码：56 / 68
页数：13
相关论文
共 59 条
  • [21] Alkaline-protease from Bacillus licheniformis MP1: Purification, characterization and potential application as a detergent additive and for shrimp waste deproteinization
    Jellouli, Kemel
    Ghorbel-Bellaaj, Olfa
    Ben Ayed, Hanen
    Manni, Laila
    Agrebi, Rym
    Nasri, Moncef
    [J]. PROCESS BIOCHEMISTRY, 2011, 46 (06) : 1248 - 1256
  • [22] Stabilization method of an alkaline protease from inactivation by heat, SDS and hydrogen peroxide
    Joo, HS
    Koo, YM
    Choi, JW
    Chang, CS
    [J]. ENZYME AND MICROBIAL TECHNOLOGY, 2005, 36 (5-6) : 766 - 772
  • [23] Production of protease from a new alkalophilic Bacillus sp I-312 grown on soybean meal:: optimization and some properties
    Joo, HS
    Chang, CS
    [J]. PROCESS BIOCHEMISTRY, 2005, 40 (3-4) : 1263 - 1270
  • [24] Joshi RH, 2008, BIOTECHNOL BIOPROC E, V13, P552, DOI [10.1007/s12257-007-0211-9, 10.1007/S12257-007-0211-9]
  • [25] Kalisz H M, 1988, Adv Biochem Eng Biotechnol, V36, P1
  • [26] Studies on the influence of bacterial collagenase in leather dyeing
    Kanth, Swama V.
    Venba, R.
    Madhan, B.
    Chandrababu, N. K.
    Sadulla, S.
    [J]. DYES AND PIGMENTS, 2008, 76 (02) : 338 - 347
  • [27] SALT-TOLERANT AND THERMOSTABLE ALKALINE PROTEASE FROM BACILLUS-SUBTILIS NCIM NO 64
    KEMBHAVI, AA
    KULKARNI, A
    PANT, A
    [J]. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1993, 38 (1-2) : 83 - 92
  • [28] Eco-friendly enzymatic dehairing of skins and hides by C-brefeldianus protease
    Khandelwal, Harish B.
    More, Snehal V.
    Kalal, K. M.
    Laxman, R. Seeta
    [J]. CLEAN TECHNOLOGIES AND ENVIRONMENTAL POLICY, 2015, 17 (02) : 393 - 405
  • [29] Kumari BL, 2013, INT J LIFE SCI BIOTE, V1, P284
  • [30] KUNDU AK, 1973, J FERMENT TECHNOL, V51, P142
123456