The properties of human CD40-activated B cells as antigen-presenting cells are not affected by PGE2

Tumor vaccination represents a promising immuno-therapeutic strategy in cancer. However, the inherent ability of many tumors to evade immune responses by suppression of immune cell function represents a major barrier. Prostaglandin E-2 (PGE(2)) has been shown to be a critical tumor-derived immunosuppressive factor. It affects a broad range of immune cells including T cells, macrophages and dendritic cells (DCs). CD40-activated B cells are being studied as a potential alternative to DCs as antigen-presenting cells for immunotherapy. So far, it is not known whether PGE(2) affects their antigen presenting capacity. We, therefore, investigated the influence of PGE(2) on the phenotype, migratory potential and antigen-presenting function of CD40-activated human B cells. Here, we demonstrate that the immunostimulatory properties of CD40-activated B cells are not affected by PGE(2). These results support the use of CD40-activated B cells as cellular adjuvants, especially in settings where PGE(2) is present in the tumor microenvironment.