Comparative analysis of developing grain transcriptome reveals candidate genes and pathways improving GPC in wheat lines derived from wild emmer

被引:2
作者
Gong, Fangyi [1 ]
Huang, Lin [1 ]
Qi, Tiangang [1 ]
Tang, Guan [1 ]
Liu, Jia [1 ]
Xiang, Lan [1 ]
He, Jingshu [1 ]
Zheng, Youliang [1 ,2 ]
Liu, Dengcai [1 ,2 ]
Wu, Bihua [1 ,2 ]
机构
[1] Sichuan Agr Univ, Triticeae Res Inst, State Key Lab Crop Gene Explorat & Utilizat South, Chengdu 611130, Peoples R China
[2] Sichuan Agr Univ, Minist Educ, Key Lab Crop Genet Resources & Improvement, Chengdu 611130, Peoples R China
基金
中国国家自然科学基金;
关键词
Wild emmer wheat; Common wheat; Wide hybridization; GPC; RNA-seq; Differentially expressed genes; PROTEIN-CONTENT LOCUS; ENDOPLASMIC-RETICULUM; TRITICUM-DICOCCOIDES; IDENTIFICATION; BREAD; ZINC; SENESCENCE; BARLEY; FAMILY; IRON;
D O I
10.1007/s13353-020-00588-y
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The grain protein content (GPC) in modern wheat is inherently low. Wild emmer wheat (Triticum turgidumssp.dicoccoides, 2n = 4x = 28, AABB) gene pool harbors wide genotypic variations in GPC. However, the characterization of candidate genes associated with high GPC is a challenge due to the complex characteristic of this trait. In the current study, we performed RNA-seq analysis on developing grains of wild emmer genotype D1, common wheat CN16, and their hexaploid wide hybrid BAd107-4 with contrasting GPC. We have found a total of 39,795 expressed genes on chromosomes A and B, of which 24,152 were shared between D1, CN16, and BAd107-4. From 1744 differentially expressed genes (DEGs), 1203 were downregulated and 541 were upregulated in the high GPC (D1+BAd107-4) compared with low GPC (CN16) groups. The majority of DEGs were associated with protein processing in endoplasmic reticulum, starch and sucrose metabolism, galactose metabolism, and protein export pathways. Expression levels of nine randomly selected genes were verified by qRT-PCR, which was consistent with the transcriptome data. The present database will help us to understand the potential regulation networks underlying wheat grain protein accumulation and provide the foundation for simultaneous improvement of grain protein content and yield in wheat breeding programs.
引用
收藏
页码:17 / 25
页数:9
相关论文
共 43 条
[11]   How Are Proteins Reduced in the Endoplasmic Reticulum? [J].
Ellgaard, Lars ;
Sevier, Carolyn S. ;
Bulleid, Neil J. .
TRENDS IN BIOCHEMICAL SCIENCES, 2018, 43 (01) :32-43
[12]   RAPD polymorphism of wild emmer wheat populations, Triticum dicoccoides, in Israel [J].
Fahima, T ;
Sun, GL ;
Beharav, A ;
Krugman, T ;
Beiles, A ;
Nevo, E .
THEORETICAL AND APPLIED GENETICS, 1999, 98 (3-4) :434-447
[13]   A novel highly differentially expressed gene in wheat endosperm associated with bread quality [J].
Furtado, A. ;
Bundock, P. C. ;
Banks, P. M. ;
Fox, G. ;
Yin, X. ;
Henry, R. J. .
SCIENTIFIC REPORTS, 2015, 5
[14]   A Complex of Pdi1p and the Mannosidase Htm1p Initiates Clearance of Unfolded Glycoproteins from the Endoplasmic Reticulum [J].
Gauss, Robert ;
Kanehara, Kazue ;
Carvalho, Pedro ;
Ng, Davis T. W. ;
Aebi, Markus .
MOLECULAR CELL, 2011, 42 (06) :782-793
[15]   Mapping genes for grain protein concentration and grain yield on chromosome 5B of Triticum turgidum (L.) var. dicoccoides [J].
Gonzalez-Hernandez, JL ;
Elias, EM ;
Kianian, SF .
EUPHYTICA, 2004, 139 (03) :217-225
[16]   Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase [J].
Harding, HP ;
Zhang, YH ;
Ron, D .
NATURE, 1999, 397 (6716) :271-274
[17]   Mapping gene(s) for grain protein in tetraploid wheat (Triticum turgidum L.) using a population of recombinant inbred chromosome lines [J].
Joppa, LR ;
Du, CH ;
Hart, GE ;
Hareland, GA .
CROP SCIENCE, 1997, 37 (05) :1586-1589
[18]   A high-grain protein content locus on barley (Hordeum vulgare) chromosome 6 is associated with increased flag leaf proteolysis and nitrogen remobilization [J].
Jukanti, Aravind K. ;
Fischer, Andreas M. .
PHYSIOLOGIA PLANTARUM, 2008, 132 (04) :426-439
[19]   Quality characteristics of durum wheat lines deriving high protein from a Triticum dicoccoides (6b) substitution [J].
Kovacs, MIP ;
Howes, NK ;
Clarke, JM ;
Leisle, D .
JOURNAL OF CEREAL SCIENCE, 1998, 27 (01) :47-51
[20]   TRANSFER OF HIGH KERNEL WEIGHT AND HIGH PROTEIN FROM WILD TETRAPLOID WHEAT (TRITICUM-TURGIDUM-DICOCCOIDES) TO BREAD WHEAT (TRITICUM-AESTIVUM) USING HOMOLOGOUS AND HOMOEOLOGOUS RECOMBINATION [J].
KUSHNIR, U ;
HALLORAN, GM .
EUPHYTICA, 1984, 33 (01) :249-255