Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes

被引：14

作者：

Anupama, M. ^{[1
]}

Murgan, S. S. ^{[1
]}

Murthy, P. Balakrishna ^{[1
]}

机构：

[1] Int Inst Biotechnol & Toxicol, Dept Genet Toxicol, Padappai 601301, Tamil Nadu, India

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2008年 / 46卷 / 10期

关键词：

Broccoli flower head; Sister chromatid exchange; Human lymphocytes; Antimutagenic; Antigenotoxic; Mitomycin-C;

D O I：

10.1016/j.fct.2008.08.009

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study is a continuation of our previous work [Murugan, S.S., Balakrishnamurthy, P., Mathew, Y.J., 2007. Antimutagenic effect of broccoli flower head by the Ames Salmonella reverse mutation assay. Phytother. Res. 21, 545-547], in search of possible antimutagenic properties in broccoli flower head extracts. In the present investigation, the effect of addition of ethanol extract of broccoli flower head on mitomycin-C (MMC) induced sister chromatid exchange (SCE) in cultured human peripheral blood lymphocytes was investigated. Broccoli flower head was extracted in ethanol using either acetone or ethanol as solvents. The extract was tested at final concentrations of 200 and 400 mu g/ml culture and set for SCE assay. MMC at a concentration of 1 mu g/ml and the test concentrations of broccoli flower head were added to the culture following 48 h from the initiation of culture. Enumeration of SCE in second division mitotic cells indicated that broccoli flower head extract significantly reduced MMC induced SCEs at both the concentrations tested. This observation is in line with our earlier finding and confirms to the presence of antimutagenic principles in broccoli flower head extract. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：3351 / 3353

页数：3

共 29 条

[1]

ATSDR (Agency for Toxic Substances and Disease Registry), 1994, TOX PROF AC

[2]

Bárta I, 2006, NEOPLASMA, V53, P19

[3] Multifunctional aspects of the action of indole-3-carbinol as an antitumor agent [J].

Bradlow, HL ;

Sepkovic, DW ;

Telang, NT ;

Osborne, MP .

CANCER PREVENTION: NOVEL NUTRIENT AND PHARMACEUTICAL DEVELOPMENTS, 1999, 889 :204-213

[4] Ingestion of an isothiocyanate metabolite from cruciferous vegetables inhibits growth of human prostate cancer cell xenografts by apoptosis and cell cycle arrest [J].

Chiao, JW ;

Wu, HY ;

Ramaswamy, G ;

Conaway, CC ;

Chung, FL ;

Wang, LG ;

Liu, DL .

CARCINOGENESIS, 2004, 25 (08) :1403-1408

[5] EFFECTS OF MITOMYCIN C ON HUMAN CHROMOSOMES [J].

COHEN, MM ;

SHAW, MW .

JOURNAL OF CELL BIOLOGY, 1964, 23 (02) :386-&

[6] Broccoli sprouts: An exceptionally rich source of inducers of enzymes that protect against chemical carcinogens [J].

Fahey, JW ;

Zhang, YS ;

Talalay, P .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (19) :10367-10372

[7] Micronucleus formation and induction of apoptosis by different isothiocyanates and a mixture of isothiocyanates in human lymphocyte cultures [J].

Fimognari, C ;

Berti, F ;

Iori, R ;

Cantelli-Forti, G ;

Hrelia, P .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2005, 582 (1-2) :1-10

[8]

Gill CIR, 2004, CANCER EPIDEM BIOMAR, V13, P1199

[9] Efficacy of sulforaphane in eradicating Helicobacter pylori in human gastric xenografts implanted in nude mice [J].

Haristoy, X ;

Angioi-Duprez, K ;

Duprez, A ;

Lozniewski, A .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (12) :3982-3984

[10] SISTER-CHROMATID EXCHANGES - A REPORT OF THE GENE-TOX PROGRAM [J].

LATT, SA ;

ALLEN, J ;

BLOOM, SE ;

CARRANO, A ;

FALKE, E ;

KRAM, D ;

SCHNEIDER, E ;

SCHRECK, R ;

TICE, R ;

WHITFIELD, B ;

WOLFF, S .

MUTATION RESEARCH, 1981, 87 (01) :17-62

← 1 2 3 →