Megahertz single-particle imaging at the European XFEL

被引：66

作者：

Sobolev, Egor ^{[1
]}

Zolotarev, Serguey ^{[2
]}

Giewekemeyer, Klaus ^{[3
]}

Bielecki, Johan ^{[3
]}

Okamoto, Kenta ^{[4
]}

Reddy, Hemanth K. N. ^{[4
]}

Andreasson, Jakob ^{[5
]}

Ayyer, Kartik ^{[6
,7
]}

Barak, Imrich ^{[8
]}

Bari, Sadia ^{[9
]}

Barty, Anton ^{[6
]}

Bean, Richard ^{[3
]}

Bobkov, Sergey ^{[2
]}

Chapman, Henry N. ^{[6
,10
,11
]}

Chojnowski, Grzegorz ^{[1
]}

Daurer, Benedikt J. ^{[4
,12
]}

Doerner, Katerina ^{[3
]}

Ekeberg, Tomas ^{[4
]}

Fluckiger, Leonie ^{[13
]}

Galzitskaya, Oxana ^{[14
,15
]}

Gelisio, Luca ^{[9
]}

Hauf, Steffen ^{[3
]}

Hogue, Brenda G. ^{[16
]}

Horke, Daniel A. ^{[6
,10
]}

Hosseinizadeh, Ahmad ^{[17
]}

Ilyin, Vyacheslav ^{[2
]}

Jung, Chulho ^{[18
]}

Kim, Chan ^{[3
]}

Kim, Yoonhee ^{[3
]}

Kirian, Richard A. ^{[19
]}

Kirkwood, Henry ^{[3
]}

Kulyk, Olena ^{[5
]}

Kuepper, Jochen ^{[6
,10
,11
]}

Letrun, Romain ^{[3
]}

Loh, N. Duane ^{[12
]}

Lorenzen, Kristina ^{[3
]}

Messerschmidt, Marc ^{[20
]}

Muhlig, Kerstin ^{[4
]}

Ourmazd, Abbas ^{[17
]}

Raab, Natascha ^{[3
]}

Rode, Andrei, V ^{[21
]}

Rose, Max ^{[9
]}

Round, Adam ^{[3
]}

Sato, Takushi ^{[3
]}

Schubert, Robin ^{[3
]}

Schwander, Peter ^{[17
]}

Sellberg, Jonas A. ^{[22
]}

Sikorski, Marcin ^{[3
]}

Silenzi, Alessandro ^{[3
]}

Song, Changyong ^{[18
]}

机构：

[1] DESY, European Mol Biol Lab, Notkestr 85, D-22607 Hamburg, Germany

[2] NRC Kurchatov Inst, Pl Akad Kurchatova 1, Moscow 123098, Russia

[3] European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany

[4] Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3 Box 596, SE-75124 Uppsala, Sweden

[5] Acad Sci Czech Republ, ELI Beamlines, Inst Phys, Slovance 2, CZ-18221 Prague, Czech Republic

[6] DESY, Ctr Free Electron Laser Sci, Notkestr 85, D-22607 Hamburg, Germany

[7] Max Planck Inst Struct & Dynam Matter, Luruper Chaussee 149, D-22761 Hamburg, Germany

[8] SAS, Inst Mol Biol, Dubravska Cesta 21, Bratislava 84551, Slovakia

[9] DESY, Notkestr 85, D-22607 Hamburg, Germany

[10] Univ Hamburg, Hamburg Ctr Ultrafast Imaging, Luruper Chaussee 149, D-22761 Hamburg, Germany

[11] Univ Hamburg, Dept Phys, Luruper Chaussee 149, D-22761 Hamburg, Germany

[12] Natl Univ Singapore, Ctr Biolmaging Sci, Dept Biol Sci, 14 Sci Dr 4, Singapore 117557, Singapore

[13] La Trobe Univ, ARC Ctr Adv Mol Imaging, Dept Chem & Phys, Melbourne, Vic 3086, Australia

[14] Russian Acad Sci, Inst Prot Res, Lab Bioinformat & Prote, Pushchino 142290, Moscow Region, Russia

[15] Russian Acad Sci, Inst Theoret & Expt Biophys, Pushchino 142290, Moscow Region, Russia

[16] Arizona State Univ, Sch Life Sci SOLS, Tempe, AZ 85287 USA

[17] Univ Wisconsin, Dept Phys, KIRC, 3135 N Maryland Ave, Milwaukee, WI 53211 USA

[18] Pohang Univ Sci & Technol, Dept Phys, Pohang 37673, South Korea

[19] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

[20] Arizona State Univ, Biodesign Inst, Tempe, AZ 85287 USA

[21] Australian Natl Univ, Laser Phys Ctr, Res Sch Phys, Canberra, ACT 2601, Australia

[22] KTH Royal Inst Technol, AlbaNova Univ Ctr, Dept Appl Phys, Biomed & Xray Phys, SE-10691 Stockholm, Sweden

[23] Uppsala Univ, Dept Phys & Astron, Box 516, SE-75120 Uppsala, Sweden

[24] SUNY Buffalo, Dept Chem, Nat Sci Complex,Room 760, Buffalo, NY 14260 USA

[25] Leibniz Inst Expt Virol, Heinrich Pette Inst, Martinistr 52, D-20251 Hamburg, Germany

[26] Brookhaven Natl Lab, NSLS 2, Upton, NY 11772 USA

[27] Natl Res Nucl Univ, MEPhI Moscow Engn Phys Inst, Kashirskoe Shosse 31, Moscow 115409, Russia

[28] Lawrence Berkeley Natl Lab, NERSC, Berkeley, CA 94720 USA

来源：

COMMUNICATIONS PHYSICS | 2020年 / 3卷 / 01期

基金：

俄罗斯科学基金会; 欧洲研究理事会; 美国国家科学基金会; 新加坡国家研究基金会; 瑞典研究理事会;

关键词：

D O I：

10.1038/s42005-020-0362-y

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previously possible. The European XFEL is expected to provide 27,000 pulses per second, over two orders of magnitude more than any other XFEL. The increased pulse rate is a key enabling factor for single-particle X-ray diffractive imaging, which relies on averaging the weak diffraction signal from single biological particles. Taking full advantage of this new capability requires that all experimental steps, from sample preparation and delivery to the acquisition of diffraction patterns, are compatible with the increased pulse repetition rate. Here, we show that single-particle imaging can be performed using X-ray pulses at megahertz repetition rates. The results obtained pave the way towards exploiting high repetition-rate X-ray free-electron lasers for single-particle imaging at their full repetition rate.

引用

页数：11

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