Microsatellite diversity and crossover regions within homozygous and heterozygous SLA haplotypes of different pig breeds

被引：20
作者：
Ando, Asako [1 ]
Uenishi, Hirohide [2 ,3 ]
Kawata, Hisako [4 ]
Tanaka-Matsuda, Maiko [3 ,5 ]
Shigenari, Atsuko [1 ]
Flori, Laurence [6 ]
Chardon, Patrick [6 ]
Lunney, Joan K. [7 ]
Kulski, Jerzy K. [1 ,8 ]
Inoko, Hidetoshi [1 ]
机构：
[1] Tokai Univ, Sch Med, Div Basic Med Sci & Mol Med, Dept Mol Life Sci, Kanazawa, Ishikawa 2591193, Japan
[2] Natl Inst Agrobiol Sci, Div Anim Sci, Tsukuba, Ibaraki 3058602, Japan
[3] Anim Genome Res Program NIAS STAFF, Tsukuba, Ibaraki 3050854, Japan
[4] Tokai Univ, Sch Med, Teaching & Res Support Ctr, Isehara, Kanagawa 2591193, Japan
[5] STAFF Inst, Res Div 2, Tsukuba, Ibaraki 3050854, Japan
[6] INRA, Lab Mixte Radiobiol & Etude Genome, CEA DSV, IRCM, F-78352 Jouy En Josas, France
[7] USDA ARS, ANRI, Anim Parasit Dis Lab, Beltsville, MD 20705 USA
[8] Univ Western Australia, Ctr Forens Sci, Nedlands, WA 6009, Australia
来源：
IMMUNOGENETICS | 2008年 / 60卷 / 07期
基金：
日本科学技术振兴机构;
关键词：
swine major histocompatibility antigen; microsatellite marker; polymorphism; homozygote; crossover;
D O I：
10.1007/s00251-008-0289-z
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Our aim was to investigate microsatellite (MS) diversity and find crossover regions at 42 polymorphic MS loci in the swine leukocyte antigen (SLA) genomic region of 72 pigs with different well-defined homozygous and heterozygous SLA haplotypes. We analyzed the genetic polymorphisms of 42 MS markers in 23 SLA homozygous-heterozygous, common pig breeds with 12 SLA serological haplotypes and 49 National Institutes of Health (NIH) and Clawn homozygous-heterozygous miniature pigs with nine SLA serological or genotyped haplotypes including four recombinant haplotypes. In comparing the same and different haplotypes, both haplospecific patterns and allelic variations were observed at the MS loci. Some of the shared haplotype blocks extended over 2 Mb suggesting the existence of strong linkage disequilibrium (LD) in the entire SLA region. Crossover regions were easily defined by the MS markers within the class I and/or III region in the NIH and Clawn recombinant haplotypes. The present haplotype comparison shows that our set of MS markers provides a fast and cost-efficient alternative, or complementary, method to the serological or sequence-based determination of the SLA alleles for the characterization of SLA haplotypes and/or the crossover regions between different haplotypes.
引用
收藏
页码：399 / 407
页数：9
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