A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

被引:46
作者
Tian, Weimin [1 ]
Liu, Xiaoling [1 ]
Wang, Gaoting [1 ]
Liu, Chenghui [1 ]
机构
[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Key Lab Appl Surface & Colloid Chem,Minist Educ, Xian 710119, Shaanxi, Peoples R China
基金
中国国家自然科学基金;
关键词
DNA; RNA; PLATFORM;
D O I
10.1039/d0cc06034h
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.
引用
收藏
页码:13445 / 13448
页数:4
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