A hyperbranched transcription-activated CRISPR-Cas12a signal amplification strategy for sensitive microRNA sensing

被引：47

作者：

Tian, Weimin ^{[1
]}

Liu, Xiaoling ^{[1
]}

Wang, Gaoting ^{[1
]}

Liu, Chenghui ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Key Lab Appl Surface & Colloid Chem,Minist Educ, Xian 710119, Shaanxi, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 87期

基金：

中国国家自然科学基金;

关键词：

DNA; RNA; PLATFORM;

D O I：

10.1039/d0cc06034h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A CRISPR-Cas12a-based strategy is developed for sensitive microRNA sensing, in which hyperbranched rolling circle amplification and transcription are integrated for the activation of the trans-cleavage ability of Cas12a. The integrated triplex signal amplification endows the strategy with a low background and high sensitivity. This design expands the scope of the CRISPR-Cas-based sensing toolbox and shows great potential in biological and biomedical studies.

引用

页码：13445 / 13448

页数：4

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