Clinical Presentation of COVID-19 and Antibody Responses in Bangladeshi Patients Infected with the Delta or Omicron Variants of SARS-CoV-2

被引:7
作者
Ghosh, Asish Kumar [1 ]
Landt, Olfert [2 ]
Yeasmin, Mahmuda [3 ]
Sharif, Mohiuddin [4 ]
Ratul, Rifat Hossain [4 ]
Molla, Maruf Ahmed [3 ]
Nafisa, Tasnim [3 ]
Mosaddeque, Mymuna Binte [5 ]
Hosen, Nur [3 ]
Bulbul, Md. Rakibul Hassan [6 ]
Mamunur, Rashid [7 ]
Islam, Alimul [8 ]
Shakil, Shahjahan Siddike [9 ]
Kaiser, Marco [2 ]
Amin, Md. Robed [4 ]
Lytton, Simon D. [10 ]
机构
[1] Dhaka Med Coll Hosp, Dept Virol, Dhaka 1000, Bangladesh
[2] TIB Molbiol GmbH, Eresburgstr 22-23, D-12103 Berlin, Germany
[3] Natl Inst Lab Med & Referral Ctr, Dhaka 1207, Bangladesh
[4] Dhaka Med Coll Hosp, Dept Med, Dhaka 1000, Bangladesh
[5] Bangabandhu Sheikh Mujib Med Univ, Dept Virol, Dhaka 1000, Bangladesh
[6] Inst Developing Sci & Hlth Initiat, Dhaka 1216, Bangladesh
[7] Bangladesh Inst Trop Infect Dis BITID, Chittagong 4317, Bangladesh
[8] Bangladesh Agr Univ, Fac Vet Sci, Dept Microbiol & Hyg, Mymensingh 2202, Bangladesh
[9] Natl Inst Dis Chest & Hosp, Dhaka 1212, Bangladesh
[10] SeraDiaLogist, D-81545 Munich, Germany
关键词
SARS CoV-2 variants; Omicron; Delta; PCR melting curve analysis; COVID-19; symptoms; hospitalization; anti-N protein; anti-S protein;
D O I
10.3390/vaccines10111959
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The clinical presentation of COVID-19 and the specific antibody responses associated with SARS-CoV-2 variants have not been investigated during the emergence of Omicron variants in Bangladesh. The Delta and Omicron variants were identified by post-PCR melting curve analysis of the spike (S) protein receptor binding domain amplicons. Anti-S-protein immunoglobulin-G anti-nucleocapsid (N)-protein immunoglobulin-G and immunoglobulin-A levels were measured by ELISA. The Delta variant was found in 40 out of 40 (100%) SARS-CoV-2 RT-PCR positive COVID-19 patients between 13 September and 23 October 2021 and Omicron variants in 90 out of 90 (100%) RT-PCR positive COVID-19 patients between 9 January and 10 February 2022. The Delta variant associated with hospitalization (74%, 80%, and 40%) and oxygen support (60%, 57%, and 40%) in the no vaccine, dose-1, and dose-2 vaccinated cases, respectively, whereas the Omicron COVID-19 required neither hospitalization nor oxygen support (0%, p < 0.0001). Fever, cough, and breathlessness were found at a significantly higher frequency among the Delta than Omicron variants (p < 0.001). The viral RNA levels of the Delta variant were higher than that of the Omicron variants (Ct median 19.9 versus 23.85; p < 0.02). Anti-spike protein immunoglobulin-G and anti-N-protein immunoglobulin-G within 1 week post onset of Delta variant COVID-19 symptoms indicate prior SARS-CoV-2 infection. The Delta variant and Omicron BA.1 and BA.2 breakthrough infections in the Dhaka region, at 240 days post onset of COVID-19 symptoms, negatively correlated with the time interval between the second vaccine dose and serum sampling. The findings of lower anti-spike protein immunoglobulin-G reactivity after booster vaccination than after the second vaccine dose suggest that the booster vaccine is not necessarily beneficial in young Bangladeshi adults having a history of repeated SARS-CoV-2 infections.
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页数:12
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