Double-headed nucleotides in DNA-zipper structures; base-base interactions and UV-induced cross-coupling in the minor groove

被引：12

作者：

Madsen, Charlotte S. ^{[1
]}

Nielsen, Lise J. ^{[1
]}

Pedersen, Nikolai S. ^{[1
]}

Lauritsen, Anne ^{[1
]}

Nielsen, Poul ^{[1
]}

机构：

[1] Univ Southern Denmark, Dept Phys Chem & Pharm, Nucle Acid Ctr, DK-5230 Odense M, Denmark

来源：

RSC ADVANCES | 2013年 / 3卷 / 27期

基金：

新加坡国家研究基金会;

关键词：

ACID SECONDARY STRUCTURES; CYCLOBUTANE DIMER; OLIGONUCLEOTIDES; DUPLEXES; ANALOGS; 2'-DEOXYNUCLEOSIDES; NUCLEOSIDES; STABILITY; RNA;

D O I：

10.1039/c3ra40857d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Nucleic acids containing double-headed nucleotides with additional nucleobases attached to the 5'(S)-position of thymidine through a methylene linker are studied. The additional bases are oriented towards base-base interactions in the minor groove of the DNA-double helix. Two new examples with adenine or cytosine as the additional bases as well as an analogue with a 4N-methylpiperazine in the same position are introduced, and in a combined study with the original double-headed nucleotide containing two thymines, interactions between the additional nucleobases across the minor groove are detected. Finally, a duplex with two thymines in the minor groove is cross-linked using UV irradiation.

引用

页码：10696 / 10706

页数：11