Quantitative proteomic analysis of drug-induced changes in mycobacteria

被引：63
作者：
Hughes, MA
Silva, JC
Geromanos, SJ
Townsend, CA
机构：
[1] Johns Hopkins Univ, Dept Chem, Baltimore, MD 21218 USA
[2] Waters Corp, Milford, MA 01757 USA
来源：
JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 01期
关键词：
isoniazid; tuberculosis; protein profiling; liquid chromatography; mass spectrometry;
D O I：
10.1021/pr050248t
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new approach for qualitative and quantitative proteomic analysis using capillary liquid chromatography and mass spectrometry to study the protein expression response in mycobacteria following isoniazid treatment is discussed. In keeping with known effects on the fatty acid synthase II pathway, proteins encoded by the kas operon (AcpM, KasA, KasB, Accd6) were significantly overexpressed, as were those involved in iron metabolism and cell division suggesting a complex interplay of metabolic events leading to cell death.
引用
收藏
页码：54 / 63
页数：10
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