Influence of carbon source on the production of exopolysacharides by Rhizobium sullae and on the nodulation of Hedysarum coronarium L. legume

被引:8
|
作者
Razika, Gharzouli [1 ]
Amira, Benahmed [1 ]
Yacine, Benhizia [1 ]
Ammar, Benguedouar [1 ]
机构
[1] Mentouri Univ Constantine, Fac Biol, Lab Mol & Cellular Biol, Constantine, Algeria
来源
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2012年 / 6卷 / 30期
关键词
Rhizobium sullae; Hedysarum coronarium; exopolysaccharides (EPS); symbiosis; EXOPOLYSACCHARIDE; STRAIN;
D O I
10.5897/AJMR12.393
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The exopolysaccharides (EPS) produced by species of the Rhizobium genus are essential for establishing symbiotic nitrogen fixation with legumes. Rhizobium strains were isolated from the wild plant Hedysarum coronarium L. collected in Constantine, Algeria. The effect of carbon source on the production of EPS and the infectivity of the bacteria were studied for the different Rhizobium isolates. Strains were cultivated on YMA where mannitol was replaced by different sugars. Good bacterial growth was found for sorbitol, sucrose, glucose and maltose. High production of EPS is obtained in the presence of mannitol, sucrose and maltose. Infectivity of the isolates was influenced by the carbon source; high infectivity was recorded in the presence of mannitol and sucrose. A relationship between carbon source, EPS production and nodulation capacity was observed.
引用
收藏
页码:5940 / 5946
页数:7
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