Adsorption study of heme proteins on SBA-15 mesoporous silica with pore-filling models

被引：76
作者：
Miyahara, M
Vinu, A
Hossain, KZ
Nakanishi, T
Ariga, K
机构：
[1] Natl Inst Mat Sci, Int Ctr Young Scientists, Tsukuba, Ibaraki 3050044, Japan
[2] Natl Inst Mat Sci, Supermol Grp, Adv Mat Lab, Tsukuba, Ibaraki 3050044, Japan
来源：
THIN SOLID FILMS | 2006年 / 499卷 / 1-2期
关键词：
mesoporous silica; protein; adsorption; nanostructures;
D O I：
10.1016/j.tsf.2005.07.046
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
The Langmuir-type adsorption of myoglobin occurred with monolayer coverage of the inner surface mesopore channels of SBA-15. Myoglobin occupation of the pores was calculated as ca. 50% based on N-2 adsorption/desorption isotherms. Pore-filling models revealed that myoglobin molecules are well packed in the pores. The maximum adsorption was observed near the isoelectric point of myoglobin, suggesting the important role of suppression of electric repulsion between the proteins and/or between the protein and the adsorbent. FT-IR spectroscopic studies confirmed that the myoglobin is stable even after the adsorption. The results obtained are comparable with those observed for another redox protein, cytochrome c. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：13 / 18
页数：6
相关论文
共 57 条
[1]   Supported membrane nanodevices [J].
Anrather, D ;
Smetazko, M ;
Saba, M ;
Alguel, Y ;
Schalkhammer, T .
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2004, 4 (1-2) :1-22
[2]   Template-assisted nano-patterning: From the submicron scale to the submolecular level [J].
Ariga, K .
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2004, 4 (1-2) :23-34
[3]   Silica-supported biomimetic membranes [J].
Ariga, K .
CHEMICAL RECORD, 2004, 3 (06) :297-307
[4]  
Ariga K, 2003, STUD SURF SCI CATAL, V146, P427
[5]   Proteosilica a novel nanocomposite with peptide assemblies in silica nanospace: Photoisomerization of spiropyran doped in chiral environment [J].
Ariga, Katsuhiko ;
Aimiya, Takuji ;
Zhang, Qingmin ;
Okabe, Akihiro ;
Niki, Makiko ;
Aida, Takuzo .
International Journal of Nanoscience, Vol 1, Nos 5 and 6, 2002, 1 (5-6) :521-525
[6]   THE DETERMINATION OF PORE VOLUME AND AREA DISTRIBUTIONS IN POROUS SUBSTANCES .1. COMPUTATIONS FROM NITROGEN ISOTHERMS [J].
BARRETT, EP ;
JOYNER, LG ;
HALENDA, PP .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1951, 73 (01) :373-380
[7]   Adsorption of gases in multimolecular layers [J].
Brunauer, S ;
Emmett, PH ;
Teller, E .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 :309-319
[8]   Characterization of polyelectrolyte-protein multilayer films by atomic force microscopy, scanning electron microscopy, and Fourier transform infrared reflection-absorption spectroscopy [J].
Caruso, F ;
Furlong, DN ;
Ariga, K ;
Ichinose, I ;
Kunitake, T .
LANGMUIR, 1998, 14 (16) :4559-4565
[9]   Ordered nanoporous polymer-carbon composites [J].
Choi, M ;
Ryoo, R .
NATURE MATERIALS, 2003, 2 (07) :473-476
[10]   Functional analogues of cytochrome c oxidase, myoglobin, and hemoglobin [J].
Collman, JP ;
Boulatov, R ;
Sunderland, CJ ;
Fu, L .
CHEMICAL REVIEWS, 2004, 104 (02) :561-588
123456