Thermoresponsive Poly(N-isopropylacrylamide)-Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication

被引：57
|
作者：
Nakayama, Masamichi [1 ]
Yamada, Naoko [2 ]
Kumashiro, Yoshikazu [1 ]
Kanazawa, Hideko [2 ]
Yamato, Masayuki [1 ]
Okano, Teruo [1 ]
机构：
[1] Tokyo Womens Med Univ TWIns, Inst Adv Biomed Engn & Sci, Shinjuku Ku, Tokyo 1628666, Japan
[2] Keio Univ, Grad Sch Pharmaceut Sci, Minato Ku, Tokyo 1058512, Japan
来源：
MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 06期
关键词：
adhesion; block copolymers; cells; poly(N-isopropylacrylamide); spin coating; RAFT POLYMERIZATION; SURFACES; DETACHMENT; CULTURE; POLYMERS; BRUSHES; HARVEST; LAYER;
D O I：
10.1002/mabi.201200018
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Thermoresponsive surfaces are prepared via a spin-coating method with a block copolymer consisting of poly(N-isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm-based polymers from the surface. The polymer coating affects the temperature-dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA-b-PIPAAm-coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer-coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.
引用
收藏
页码：751 / 760
页数：10
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