Cloning, expression and characterization of a novel chitosanase from Streptomyces albolongus ATCC 27414

被引：57

作者：

Guo, Na ^{[1
]}

Sun, Jianan ^{[1
]}

Wang, Wei ^{[1
]}

Gao, Li ^{[1
]}

Liu, Jinbao ^{[1
]}

Liu, Zhen ^{[1
]}

Xue, Changhu ^{[1
,2
]}

Mao, Xiangzhao ^{[1
,2
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

[2] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China

来源：

FOOD CHEMISTRY | 2019年 / 286卷

关键词：

Chitosan; Chitosanase; GH; 46; family; Streptomyces albolongus; BETA-D-GLUCOSAMINIDASE; PURIFICATION; CHITOOLIGOSACCHARIDES; GENE; STABILITY; CHITIN; FUNGUS; MODE; N174;

D O I：

10.1016/j.foodchem.2019.02.056

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A gene encoding chitosanase from Streptomyces albolongus was cloned, sequenced and expressed in Escherichia coli. The novel recombinant enzyme (Csn21c) was purified by Ni-NTA Superflow Column and showed a molecular mass of 29.6 kDa by SDS-PAGE. The enzyme Csn21c showed the optimal activity in 50 mmol/L Tris-HCl buffer, pH 8.0, and 50 degrees C and it was strongly activated (2-fold) by Mn2+. It belonged to glycoside hydrolase 46 family according to NCBI database (http://www.ncbi.nlm.nih.gov/) and displayed an exo-type cleavage pattern, hydrolyzing chitosan mainly into D-glucosamine (GlcN) and chitobiose ((GlcN)(2)) as confirmed by TLC and MS analysis. This study demonstrated that Csn21c can be an effective tool to produce abundant glucosamine and chitooligosaccharides (COS) from chitosan.

引用

页码：696 / 702

页数：7

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