Arthrobacter paludis sp nov., isolated from a marsh

A novel Gram-stain-positive, strictly aerobic, non-endospore-forming bacterium, designated CAU 9143(T), was isolated from a hydric soil sample collected from Seogmo Island in the Republic of Korea. Strain CAU 9143(T) grew optimally at 30 degrees C, at pH 7.0 and in the presence of 1% (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences revealed that strain CAU 9143(T) belonged to the genus Arthrobacter and was closely related to Arthrobacter ginkgonis SYP-A7299(T) (97.1% similarity). Strain CAU 9143(T) contained menaquinone MK-9 (H-2) as the major respiratory quinone and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and two unidentified phospholipids as the major polar lipids. The whole-cell sugars were glucose and galactose. The peptidoglycan type was A4a (L-Lys-D-Glu2) and the major cellular fatty acid was anteiso-C-15 : 0. The DNA G+C content was 64.4 mol% and the level of DNA-DNA relatedness between CAU 9143(T) and the most closely related strain, A. ginkgonis SYP-A7299(T), was 22.3 %. Based on phenotypic, chemotaxonomic and genetic data, strain CAU 9143(T) represents a novel species of the genus Arthrobacter, for which the name Arthrobacter paludis sp. nov. is proposed. The type strain is CAU 9143(T) (=KCTC 13958(T),=CECT 8917(T)).