Outcome of adrenal tissue fragments allotransplantation: The impact of cryopreservation

被引：2

作者：

Alabedalkarim, N. M. ^{[1
]}

Bozhok, G. A. ^{[1
]}

Legach, E. I. ^{[1
]}

Ustichenko, V. D. ^{[1
]}

Zubov, P. M. ^{[1
]}

Bilyavskaya, S. B. ^{[1
]}

Dudetskaya, G. V. ^{[1
]}

Bondarenko, T. P. ^{[1
]}

Hoffmann, M. W. ^{[2
]}

机构：

[1] Natl Acad Sci Ukraine, Inst Problems Cryobiol & Cryomed, Kharkov, Ukraine

[2] Hannover Med Sch, Dept Visceral & Transplantat Surg, D-3000 Hannover, Germany

来源：

CRYOBIOLOGY | 2012年 / 65卷 / 03期

关键词：

Cryopreservation; DMSO; Adrenal glands; Transplantation; Dendritic cells; Macrophages; Corticosterone; IMMUNE-ENDOCRINE INTERACTIONS; MHC CLASS-II; PANCREATIC-ISLETS; DENDRITIC CELLS; TESTICULAR MACROPHAGES; MONOCLONAL-ANTIBODIES; ADRENALECTOMIZED RATS; ADRENOCORTICAL-CELLS; MEMBRANE INTEGRITY; ADDISONS-DISEASE;

D O I：

10.1016/j.cryobiol.2012.05.013

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cryopreservation is thought to have the potential to preserve tissue for transplantation. In addition, it can also be used for decreasing tissue immunogenicity, which might be important for prolonging allograft survival. In the present study we examined the impact of cryopreservation at various cooling rates on the outcome of allotransplantation of murine adrenal tissue fragments (ATFr). ATFr were cryopreserved with a cooling rate at 1; 10; 40 and more than 100 degrees C/min. After thawing it was found that the number of the cells expressing markers of dendritic cells (CD11c) and macrophages (CD11b) in the suspension obtained from ATFr decreased with increasing cooling rate. After allotransplantation the survival rates of adrenalectomized mice and the blood serum levels of corticosterone were higher in recipients of cryopreserved ATFr. By immunohistochemistry, cryopreserved allografts displayed a decreased infiltration by CD4(+) and CD8(+) T-lymphocytes as compared to fresh grafts. These findings suggest that cryopreserved allografts cause a less severe rejection by decreasing graft immunogenicity. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：188 / 195

页数：8

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