Characterization of purified and ex vivo manipulated human hematopoietic progenitor and stem cells in xenograft recipients

被引：6

作者：

Bock, TA ^{[1
]}

Ziegler, BL ^{[1
]}

Bühring, HJ ^{[1
]}

Scheding, S ^{[1
]}

Brugger, W ^{[1
]}

Kanz, L ^{[1
]}

机构：

[1] Univ Tubingen, Dept Hematol & Oncol, D-72076 Tubingen, Germany

来源：

HEMATOPOIETIC STEM CELLS: BIOLOGY AND TRANSPLANTATION | 1999年 / 872卷

关键词：

D O I：

10.1111/j.1749-6632.1999.tb08465.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Research on the biology, regulation, and transplantation of hu man hematopoietic stem cells requires test systems for the detection, monitoring, and quantitation of these cells. Xenografted animal models provide suitable stem cell assays, since they allow long-term engraftment, multilineage differentiation, and serial transfer of human hematopoietic cells. Recent techniques for the separation of hematopoietic cells have provided highly purified cellular subsets selected on the basis of the surface marker phenotype. The stem cell content of these subsets, however, is still unclear. Also, innovative approaches for the induction of hematopoietic cell proliferation and differentiation have generated ex vivo manipulated cells whose biological properties and functions still remain to be assessed. This paper reports on the biological characterization of these cell populations by the use of xenograft models.

引用

页码：200 / 210

页数：11

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