Combinatorial evaluation of laccase-mediator system in the oxidation of veratryl alcohol

被引：20

作者：

Larson, Troy M. ^{[1
]}

Anderson, Amber M. ^{[1
]}

Rich, Joseph O. ^{[1
]}

机构：

[1] ARS, USDA, Renewable Prod Technol Res Unit, Natl Ctr Agr Utilizat Res, Peoria, IL 61604 USA

来源：

BIOTECHNOLOGY LETTERS | 2013年 / 35卷 / 02期

关键词：

Laccase; Laccase mediator system; Mediator; Veratraldehyde; Veratryl alcohol oxidation; FUNGAL LACCASES; PURIFICATION;

D O I：

10.1007/s10529-012-1078-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. Screening revealed complete conversion of veratryl alcohol to veratraldehyde catalyzed by a crude preparation of the laccase from Trametes versicolor ATCC 11235 and the mediator TEMPO in 20 % (v/v) tert-butanol.

引用

页码：225 / 231

页数：7