共 21 条
- [21] New Treatments for Bacterial Keratitis[J]. JOURNAL OF OPHTHALMOLOGY, 2012, 2012Wong, Raymond L. M.Gangwani, R. A.Yu, Lester W. H.Lai, Jimmy S. M.
Effect of Photodynamic Inactivation (PDI) using Riboflavin-Conjugated Antibody against Staphylococcus aureus
被引：2
作者：
Song, X.
[1
]
Stachon, T.
[1
]
Seitz, B.
[1
]
Wang, J.
[1
]
Bischoff, M.
[2
]
Langenbucher, A.
[3
]
Janunts, E.
[3
]
Szentmary, N.
[1
]
机构：
[1] Univ Klinikum Saarlandes UKS, Klin Augenheilkunde, D-66424 Homburg, Germany
[2] Univ Klinikum Saarlandes UKS, Inst Mikrobiol & Hyg, D-66424 Homburg, Germany
[3] Univ Klinikum Saarlandes UKS, Expt Ophthalmol, D-66424 Homburg, Germany
关键词：
crosslinking;
photodynamic inactivation;
Staphylococcus aureus;
riboflavin-conjugated antibody;
COLLAGEN CROSS-LINKING;
THERAPY;
BACTERIAL;
CHARGE;
D O I：
10.1055/s-0035-1545834
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
Purpose: Crosslinking/riboflavin-UVA photodynamic therapy is a potential treatment alternative in antibiotic resistant infectious keratitis. For photodynamic therapy a specific (against bacteria) conjugated antibody may be used in order to increase the effect of the treatment. In our present study we analysed the impact of photodynamic inactivation using riboflavin-conjugated antibody or riboflavin alone on Staphylococcus aureus, in vitro. Methods: Staphylococcus aureus (S. aureus) was incubated in 1:100 diluted riboflavin-conjugated antibody (R-AB) for 30 minutes in darkness. Following UVA-light illumination (375nm) with an energy dose of 2, 3, 4 and 8 J/cm(2), bacteria were brought to blood agar Plates for 24 hours before colony-forming unit (CFU) counting. In an additional group, we incubated bacteria to 0, 0.05 or 0.1% riboflavin 5-phosphate as described above followed by illumination using UVA light (375nm) with an energy dose of 2 J/cm(2), before CFU counting. Results: The number of CFU decreased significantly (inactivation of 36%, p=0.022) using 1:100 diluted riboflavin-conjugated antibody and 2 J/cm(2)UVA-light illumination, compared to untreated controls. The use of 3, 4 und 8 J/cm(2) energy dose and R-AB in 1:100 dilution did not further change the decrease of CFU (inactivation of 39, 39 and 40%; p=0.016; p=0.016; p=0.015). The use of 0.05% or 0.1% riboflavin 5-phosphate alone and UVA-light illumination reduced the CFU count significantly (inactivation of 73 and 55%; p=0.002; p=0.005), compared to untreated controls. Conclusions: The use of riboflavin-conjugated antibody or 0.05% or 0.1% riboflavin 5-phosphate and UVA-light illumination reduces the number of CFU of S. aureus. However, none of these photodynamic therapies reached the necessary 99% killing rate of these bacteria. Further work is needed to increase the efficacy of riboflavin-conjugated antibodies against antibiotic resistant bacteria.
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页码：988 / 992
页数：5
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