Effects of dietary Bacillus natto supplementation on growth performance and the growth-related gene/microRNA expression in the skeletal muscle of grass carp (Ctenopharyngodon idella)

被引：12

作者：

Li, Z. J. ^{[1
]}

Chen, Y. H. ^{[1
]}

Zhang, J. Z. ^{[2
]}

Zhu, X. ^{[2
]}

Zhang, J. S. ^{[3
]}

Chen, D. X. ^{[3
]}

Wang, K. Z. ^{[3
]}

Hu, Y. ^{[2
]}

Chu, W. Y. ^{[1
]}

机构：

[1] Hunan Agr Univ, Coll Food Sci & Technol, Changsha, Hunan, Peoples R China

[2] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China

[3] Changsha Univ, Dept Bioengneering & Environm Sci, Changsha, Hunan, Peoples R China

来源：

AQUACULTURE NUTRITION | 2017年 / 23卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Bacillus natto; gene expression; grass carp; growth performance; microRNA; skeletal muscle; GENE-EXPRESSION; LITOPENAEUS-VANNAMEI; WHITE SHRIMP; PROLIFERATION; ENHANCEMENT; IMPROVEMENT; MICRORNA-1; PROBIOTICS; MYOSTATIN; IMMUNITY;

D O I：

10.1111/anu.12357

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

In this study, Bacillus natto NT was evaluated for use as a probiotic supplement in the feeds on the growth performance and the growth-related genes' and microRNAs' (miRNAs) expression in the skeletal muscle of grass carp (Ctenopharyngodon idella). Grass carps (ave. wt. 43.96 +/- 0.27 g) were fed diets supplemented with 1.87 9 10 9 (Bn1), 3.73 +/- 10(9) (Bn2), 5.60 x 10(9) (Bn3), 7.47 x 10(9) (Bn4) and 9.33 x 10(9) (Bn5) B. natto NT cells per 100 g feed for 56 days. The control group (BnC) was not supplemented with the B. natto NT. The fish of Bn3, Bn4 and Bn5 groups displayed better growth performance and lower feed conversion ratio (FCR) than the other groups (P < 0.05). Compared with the fish of BnC group, miR-1a, miR-181a, miR-23a and miR-206 expressions increased (P < 0.05), and the myostatin and myocyte enhancer factor C (MEF2C) mRNAs were down-regulated (P < 0.05) in the fish of Bn4 and Bn5 groups. The specific growth rate analysis and apparent expressional regulation of the growth-related miRNAs and genes stimulated by B. natto suggest the potential application of B. natto in improving the growth performance on the grass carps.

引用

页码：46 / 53

页数：8

共 33 条

[1] Lack of myostatin results in excessive muscle growth but impaired force generation [J].

Amthor, Helge ;

Macharia, Raymond ;

Navarrete, Roberto ;

Schuelke, Markus ;

Brown, Susan C. ;

Otto, Anthony ;

Voit, Thomas ;

Muntoni, Francesco ;

Vrbova, Gerta ;

Partridge, Terence ;

Zammit, Peter ;

Bunger, Lutz ;

Patel, Ketan .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (06) :1835-1840

[2] The effect of lactic acid bacteria administration on growth, digestive enzyme activity and gut microbiota in Persian sturgeon (Acipenser persicus) and beluga (Huso huso) fry [J].

Askarian, F. ;

Kousha, A. ;

Salma, W. ;

Ringo, E. .

AQUACULTURE NUTRITION, 2011, 17 (05) :488-497

[3] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2009, 55 (04) :611-622

[4] The Polycomb EA2 methyltransferase regulates muscle gene expression and skeletal muscle differentiation [J].

Caretti, G ;

Di Padova, M ;

Micales, B ;

Lyons, GE ;

Sartorelli, V .

GENES & DEVELOPMENT, 2004, 18 (21) :2627-2638

[5] Growth improvement by probiotic in European sea bass juveniles (Dicentrarchus labrax, L.), with particular attention to IGF-1, myostatin and cortisol gene expression [J].

Carnevali, Oliana ;

de Vivo, Luisa ;

Sulpizio, Roberto ;

Gioacchini, Giorgia ;

Olivotto, Ike ;

Silvi, Stefania ;

Cresci, Alberto .

AQUACULTURE, 2006, 258 (1-4) :430-438

[6] An evaluation of two probiotic bacterial strains, Enterococcus faecium SF68 and Bacillus toyoi, for reducing edwardsiellosis in cultured European eel, Anguilla anguilla L. [J].

Chang, CI ;

Liu, WY .

JOURNAL OF FISH DISEASES, 2002, 25 (05) :311-315

[7] The role of microRNA-1 and microRNA-133 in skeletal muscle proliferation and differentiation [J].

Chen, JF ;

Mandel, EM ;

Thomson, JM ;

Wu, QL ;

Callis, TE ;

Hammond, SM ;

Conlon, FL ;

Wang, DZ .

NATURE GENETICS, 2006, 38 (02) :228-233

[8] Effects of dietary lactosucrose on the gene transcript profile in liver of grass carp (Ctenopharyngodon idella) [J].

Chu, W. Y. ;

Liu, X. L. ;

Chen, D. X. ;

Shi, J. ;

Chen, Y. H. ;

Li, Y. L. ;

Zeng, G. Q. ;

Wu, Y. A. ;

Zhang, J. S. .

AQUACULTURE NUTRITION, 2013, 19 (05) :798-808

[9] Systematic Identification and Differential Expression Profiling of MicroRNAs from White and Red Muscles of Siniperca chuatsi [J].

Chu, W. -Y. ;

Liu, L. -S. ;

Li, Y. -L. ;

Chen, L. ;

Wang, K. -Z. ;

Li, H. -H. ;

Du, S. -J. ;

Zhang, J. -S. .

CURRENT MOLECULAR MEDICINE, 2013, 13 (08) :1397-1407

[10] Molecular cloning and mRNA expression analysis of myosin heavy chain (MyHC) from fast skeletal muscle of grass carp, Ctenopharyngodon idella [J].

Chu Wuying ;

Fu Guihong ;

Bing Shiyu ;

Meng Tao ;

Zhou Ruixue ;

Cheng Jia ;

Zhao Falan ;

Zhang Hongfang ;

Zhang Jianshe .

CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY, 2010, 28 (02) :239-247

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