Application of an enzyme encapsulated metal-organic framework composite for convenient sensing and degradation of methyl parathion

被引：55
作者：
Mehta, Jyotsana [1 ,2 ]
Dhaka, Sarita [1 ]
Bhardwaj, Neha [1 ,2 ]
Paul, Ashok K. [3 ]
Dayananda, Siddavattam [4 ]
Lee, Sung-Eun [5 ]
Kim, Ki-Hyun [6 ]
Deep, Akash [1 ]
机构：
[1] CSIR, CSIO, Nanosci & Nanotechnol Lab, Sect 30 C, Chandigarh 160030, India
[2] CSIR, CSIO, Acad Sci & Innovat Res, Sect 30 C, Chandigarh 160030, India
[3] Desh Bhagat Univ, Mandi Gobindgarh 147301, Punjab, India
[4] Univ Hyderabad, Sch Life Sci, Hyderabad 500046, India
[5] Kyungpook Natl Univ, Sch Appl Biosci, Daegu 41566, South Korea
[6] Hanyang Univ, Dept Civil & Environm Engn, 222 Wangsimni Ro, Seoul 04763, South Korea
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 290卷
基金：
新加坡国家研究基金会;
关键词：
Organophosphates; Methyl parathion; Organophosphate hydrolase; MOF; Sensing; Degradation; ORGANOPHOSPHATE PESTICIDES; COORDINATION MODULATION; BIOREMEDIATION; HYDROLASE; BIOSENSOR; WATER; MOF; IMMOBILIZATION; FABRICATION; CARBON;
D O I：
10.1016/j.snb.2019.03.116
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Terbium-BTC (BTC= 1,2,4-Benzenetricarboxylic acid) metal-organic framework (MOF) has been employed for in-situ encapsulation of hexahistidine-tagged organophosphate hydrolase (OPH6His) enzyme. The enzymatic activity and stability of OPH6His were found remarkably enhanced after its encapsulation within the MOF. The potential of this enzyme-MOF composite (OPH6His@Tb-BTC) has been explored as a dual platform to simultaneously detect organophosphate pesticides (OPPs) and catalyze their degradation. The OPH6His@Tb-BTC exhibited improved enzymatic activity (by approximately 30%) and reusability (at least five cycles). It is demonstrated to be a highly sensitive and simple optical bioprobe for the detection of an OPP candidate, methyl parathion with a low limit of detection (2.6 nM). A less toxic metabolite was formed through catalytic conversion of methyl parathion (i.e., upon its incubation with OPH6His@Tb-BTC), further supporting the promising role of this composite in the remediation of pesticide-contaminated environments.
引用
收藏
页码：267 / 274
页数：8
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