Pattern of expression of beta-defensins in oral squamous cell carcinoma

被引：67

作者：

Abiko, Y ^{[1
]}

Mitamura, J

Nishimura, M

Muramatsu, T

Inoue, T

Shimono, M

Kaku, T

机构：

[1] Hlth Sci Univ Hokkaido, Dept Oral Pathol, Sch Dent, Ishikari, Hokkaido 0610293, Japan

[2] Tokyo Denki Coll, Dept Pathol, Mihama Ku, Chiba, Japan

来源：

CANCER LETTERS | 1999年 / 143卷 / 01期

关键词：

defensin; squamous cell carcinoma; oral; reverse transcriptase-polymerase chain reaction; antimicrobial peptide;

D O I：

10.1016/S0304-3835(99)00171-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Human beta-defensin (hBD)-1 and hBD-2 are antimicrobial peptides that have been detected in certain types of epithelia, including the skin and oral epithelia. It has been suggested that bacterial infection is an important factor in the process of carcinogenesis. The expression of hBDs in oral squamous cell carcinoma (SCC) may be down-regulated. We studied the pattern of expression of hBD-1 and hBD-2 mRNA in oral (SCC) cell lines and in tumor samples obtained from four patients with oral SCC who underwent surgical resection, by reverse transcription-polymerase chain reaction (RT-PCR). Human gingival epithelial (HGE) cells were used as the control. The effect of various inflammatory cytokines on hBD-1 and hBD-2 expression in the HGE cells and SCC cell lines, was also studied. hBD-1 mRNA was detected in the Ca-9, SCC-9 and HSC-4 cell lines, but not in the SAS and KB cell lines. hBD-2 mRNA was detected in all five cell lines. All four tumor samples expressed both hBD-1 and kBD-2 mRNA, although the mRNA level of each protein varied. These results indicate that SCCs in which hBD expression is downregulated, may be susceptible to bacterial infection. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：37 / 43

页数：7