Novel method for screening Saccharomyces cerevisiae mutants with increased sulfur-containing compounds: Color-based selection of colonies using the met30 strain

被引：2

作者：

Tarutina, Marina G. ^{[1
]}

Dutova, Tatiana A. ^{[1
]}

Yezhova, Inna E. ^{[1
]}

Nishiuchi, Hiroaki ^{[2
]}

Sineoky, Sergey P. ^{[1
]}

机构：

[1] State Res Inst Genet & Select Ind Microorganisms, Russian State Collect Ind Microorganisms VKPM, Moscow 117545, Russia

[2] Ajinomoto Co Inc, Kawasaki Ku, Kawasaki, Kanagawa 2108681, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2012年 / 114卷 / 06期

关键词：

Visual selection of colonies; Glutathione; Yeast with red pigmentation; Saccharomyces cerevisiae; ade1; mutation; ade2; SCHIZOSACCHAROMYCES-POMBE; S-ADENOSYLMETHIONINE; RED PIGMENT; PATHWAY; GLUTATHIONE; METHIONINE; YEAST; BIOSYNTHESIS; TRANSPORT;

D O I：

10.1016/j.jbiosc.2012.07.011

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glutathione overproducers were detected by examining the pigmentation intensity of Saccharomyces cerevisiae met30 yeast carrying wild-type alleles for ADE1 and ADE2. Highly pigmented colonies, phenocopies of the ade2 or ade1 mutants, were observed among yeast grown in minimal biotin-free medium with a high methionine content. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：619 / 621

页数：3

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