Electrochemical Oxidation of Astaxanthin on Glassy-carbon Electrode and its Amperometric Determination Using Batch Injection Analysis (BIA)

This work presents the electrochemical oxidation of the antioxidant astaxanthin on a glassy-carbon electrode (GCE) and its amperometric determination in salmon samples using a batch-injection analysis (BIA) system. The proposed BIA method consisted of 80-mL a fast microliter injection of sample at 193 mL s(-1) on the GCE immersed in the electrolyte, a mixture of acetone, dichloromethane, and water (80 : 10 : 10 v/v), containing 0.1 mol L-1 HClO4. Advantages include high precision (RSD of 2.4%), sample throughput of 240 h(-1), and low detection limit (0.3 mmol L-1 that corresponds to 0.1 mu g g(-1)) for the analysis of acetone extracts of salmon samples. Recovery values between 83 and 97% attested the accuracy of the method.