Diffuse wave spectroscopy for optical properties measurements of normal and coagulated chicken liver using ultrafast femtosecond wavelength range (390-435 nm)

In this study, we have determined the optical properties (absorption coefficient, scattering coefficient, reduced scattering coefficient and penetration depth of laser radiation) of normal and thermally coagulated chicken liver at wavelengths of 390, 395, 400, 405, 410, 415, 420, 425, 430 and 435 nm of laser irradiation using the Kubelka Munk model from the radial dependence of diffused wave spectroscopy data in the femtosecond short pulsed-laser regime. We have also proved that the diffused signal is not affected by up to 1 minute of data collection from the liver tissue. The absorption and scattering coefficients have been observed to be significantly increased due to the coagulation of the chicken liver at 90 degrees C. An increasing trend in the calculated values of optical parameters of normal and abnormal chicken liver within 390 nm-435 nm wavelength range has been observed. The total attenuation coefficient has been calculated with the help of absorption and scattering coefficient. We believe that these significant differences in optical properties will be helpful to understand the optimal use of laser application and diagnosis of tissue for different optical therapeutic techniques like photodynamic therapy. This technique can be extended for optical parameter measurements of superficial organs e.g. breast cancer and skin tumors.