In vitro development of human primordial follicles to preantral stage after vitrification

被引：19
作者：
Khosravi, Fariba [1 ,3 ]
Reid, Robert L. [2 ]
Moini, Ashraf [4 ]
Abolhassani, Farid [3 ]
Valojerdi, Mojtaba R. [5 ]
Kan, Frederick W. K. [1 ]
机构：
[1] Queens Univ, Dept Biomed & Mol Sci, Fac Hlth Sci, Kingston, ON K7L 3N6, Canada
[2] Queens Univ, Dept Obstet & Gynecol, Fac Hlth Sci, Kingston, ON K7L 3N6, Canada
[3] Univ Tehran Med Sci, Dept Anat, Sch Med, Tehran, Iran
[4] Univ Tehran Med Sci, Dept Obstet & Gynecol, Sch Med, Tehran, Iran
[5] Tarbiat Modares Univ, Dept Anat, Fac Med Sci, Tehran, Iran
来源：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 2013年 / 30卷 / 11期
基金：
加拿大健康研究院;
关键词：
Carrier device; In vitro culture; Preantral follicles; Preservation of fertility; Primordial follicles; HUMAN OVARIAN TISSUE; SOLID-SURFACE VITRIFICATION; PRE-ANTRAL FOLLICLES; SERUM-FREE CULTURE; FOLLICULAR-GROWTH; VITRIFIED OVARIES; CRYOPRESERVATION; MOUSE; TRANSPLANTATION; MATURATION;
D O I：
10.1007/s10815-013-0105-z
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Purpose The aim was to culture primordial follicles in vitro to reach preantral stage in vitrified human ovarian tissue. Methods Ovarian tissue samples were obtained from six women. Tissue strips were vitrified by infiltration with a cryoprotectant followed by mounting on a stainless steel carrier. After culturing for 7 days the morphology and developmental stages of follicles enclosed in fresh and vitrified groups were analyzed. Results High proportion of viable follicles in vitrified ovarian strips was obtained. After culturing for 7 days the percentage of secondary and preantral follicles increased significantly (P<0.05) whereas primordial and transitory follicles showed a significant decrease (P<0.05) compared to their respective counterparts at day 0 of culture. Conclusions Vitrification of ovarian strips with an improved carrier device and culturing of follicles in ovarian strips after warming yielded developed follicles with high viability and morphological integrity that may be suitable for use in fertility preservation among cancer patients.
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收藏
页码：1397 / 1406
页数：10
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