Heat shock protein 20 (HSP20) is a novel substrate for protein kinase D1 (PKD1)

被引：11
作者：
Sin, Yuan Yan [1 ]
Baillie, George S. [1 ]
机构：
[1] Univ Glasgow, CMVLS, Inst Cardiovasc & Med Sci, Glasgow G12 8QQ, Lanark, Scotland
来源：
CELL BIOCHEMISTRY AND FUNCTION | 2015年 / 33卷 / 07期
基金：
英国惠康基金;
关键词：
HSP20; PKD1; PKA; peptide array; cardiac remodelling; CARDIAC TROPONIN-I; HISTONE DEACETYLASE-5; BETA-ARRESTIN; C-MU; PHOSPHORYLATION; ACTIVATION; APOPTOSIS; HYPERTROPHY; INHIBITION; RELAXATION;
D O I：
10.1002/cbf.3147
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Heat shock protein 20 (HSP20) has cardioprotective qualities, which are triggered by PKA phosphorylation. PKD1 is also a binding partner for HSP20, and this prompted us to investigate whether the chaperone was a substrate for PKD1. We delineate the PKD1 binding sites on HSP20 and show for the first time HSP20 is a substrate for PKD1. Phosphorylation of HSP20 by PKD1 is diminished by pharmacological or siRNA reduction of PKD1 activity and is enhanced following PKD1 activation. Our results suggest that both PKA and PKD1 can both phosphorylate HSP20 on serine 16 but that PKA is the most dominant. Copyright (c) 2015 John Wiley & Sons, Ltd.
引用
收藏
页码：421 / 426
页数：6
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