Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica

被引：23
|
作者：
Jirku, Milan
Zemanova, Eva
Al-Jawabreh, Amer
Schoenian, Gabriele
Lukes, Julius [1 ]
机构：
[1] Acad Sci Czech Republ, Inst Parasitol, CR-37005 Ceske Budejovice, Czech Republic
[2] Univ S Bohemia, Fac Biol, Ceske Budejovice 37005, Czech Republic
[3] Humboldt Univ, Charite, Inst Microbiol & Hyg, D-10117 Berlin, Germany
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2006年 / 55卷 / 01期
关键词：
kinetoplastids; Leishmania tropica; PCR assay; differential diagnosis;
D O I：
10.1016/j.diagmicrobio.2005.12.001
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
We have developed a polymerase chain reaction assay for differential diagnosis of Leishmania tropica, based on simple amplification of the target region. The assay detects less than 5 protozoan cells, was tested on human samples and an experimentally infected animal, and is appropriate for clinical laboratories in countries where leishmaniasis is endemic. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码：75 / 79
页数：5
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