A high-throughput three-dimensional cell migration assay for toxicity screening with mobile device-based macroscopic image analysis

被引：75

作者：

Timm, David M. ^{[1
,2
]}

Chen, Jianbo ^{[1
,2
]}

Sing, David ^{[2
,3
]}

Gage, Jacob A. ^{[2
]}

Haisler, William L. ^{[2
,3
]}

Neeley, Shane K. ^{[2
,3
]}

Raphael, Robert M. ^{[3
]}

Dehghani, Mehdi ^{[4
]}

Rosenblatt, Kevin P. ^{[4
]}

Killian, T. C. ^{[1
]}

Tseng, Hubert ^{[2
]}

Souza, Glauco R. ^{[2
]}

机构：

[1] Rice Univ, Dept Phys, Houston, TX 77005 USA

[2] Nano3D Biosci N3D, Houston, TX 77030 USA

[3] Rice Univ, Dept Bioengn, Houston, TX 77005 USA

[4] Univ Texas Hlth Sci Ctr Houston, Brown Fdn Inst Mol Med Prevent Human Dis, Houston, TX 77030 USA

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

基金：

美国国家科学基金会;

关键词：

CULTURE; MATRIX; NANOPARTICLES; BACTERIOPHAGE; MODEL;

D O I：

10.1038/srep03000

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

There is a growing demand for in vitro assays for toxicity screening in three-dimensional (3D) environments. In this study, 3D cell culture using magnetic levitation was used to create an assay in which cells were patterned into 3D rings that close over time. The rate of closure was determined from time-lapse images taken with a mobile device and related to drug concentration. Rings of human embryonic kidney cells (HEK293) and tracheal smooth muscle cells (SMCs) were tested with ibuprofen and sodium dodecyl sulfate (SDS). Ring closure correlated with the viability and migration of cells in two dimensions (2D). Images taken using a mobile device were similar in analysis to images taken with a microscope. Ring closure may serve as a promising label-free and quantitative assay for high-throughput in vivo toxicity in 3D cultures.

引用

页数：8

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