Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by Mass Spectrometry

被引:23
作者
Wandernoth, Petra [1 ]
Kriegsmann, Katharina [2 ]
Groh-Mohanu, Cristina [1 ]
Daeumer, Martin [3 ]
Gohl, Peter [4 ]
Harzer, Oliver [4 ]
Kriegsmann, Mark [5 ,6 ]
Kriegsmann, Joerg [1 ,7 ]
机构
[1] Ctr Histol Cytol & Mol Diagnost Trier, D-54296 Trier, Germany
[2] Univ Hosp Heidelberg, Dept Hematol Oncol & Rheumatol, D-69120 Heidelberg, Germany
[3] Kaiserslautern Med Lab, Inst Immunol & Genet, D-67655 Kaiserslautern, Germany
[4] Bioscientia, D-576080 Ingelheim, Germany
[5] Univ Hosp Heidelberg, Inst Pathol, D-69120 Heidelberg, Germany
[6] German Ctr Lung Canc Res DZL, D-69120 Heidelberg, Germany
[7] Danube Private Univ Krems, A-3500 Krems, Austria
来源
VIRUSES-BASEL | 2020年 / 12卷 / 08期
关键词
SARS-CoV2; COVID19; mass spectrometry; severe acute respiratory syndrome coronavirus 2; ROUTINE;
D O I
10.3390/v12080849
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: Amplification of viral ribonucleic acid (RNA) by real-time reverse transcriptase polymerase chain reaction (rRT-PCR) is the gold standard to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since the initial outbreak, strategies to detect and isolate patients have been important to avoid uncontrolled viral spread. Although testing capacities have been upscaled, there is still a need for reliable high throughput test systems, specifically those that require alternative consumables. Therefore, we tested and compared two different methods for the detection of viral PCR products: rRT-PCR and mass spectrometry (MS). Methods: Viral RNA was isolated and amplified from oro- or nasopharyngeal swabs. A total of 22 samples that tested positive and 22 samples that tested negative for SARS-CoV-2 by rRT-PCR were analyzed by MS. Results of the rRT-PCR and the MS protocol were compared. Results: Results of rRT-PCR and the MS test system were in concordance in all samples. Time-to-results was faster for rRT-PCR. Hands-on-time was comparable in both assays. Conclusions: MS is a fast, reliable and cost-effective alternative for the detection of SARS-CoV-2 from oral and nasopharyngeal swabs.
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页数:8
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