Determination of cefoperazone and sulbactam in serum by HPLC-MS/MS: An adapted method for therapeutic drug monitoring in children

被引：14
作者：
Wu, Xiu-Jun [1 ]
Huang, Xin [2 ]
Shi, Hai-Yan [2 ,3 ]
Chen, Xing-Kai [3 ]
Dong, Qian [3 ]
Hao, Guo-Xiang [3 ]
Li, Yan [2 ]
Zheng, Yi [3 ]
Zhao, Wei [2 ,3 ]
机构：
[1] Liaoning Univ Tradit Med, Lab Clin Pharmacokinet TCM, Affiliated Hosp, Shenyang, Liaoning, Peoples R China
[2] Shandong Univ, Shandong Prov Qianfoshan Hosp, Dept Pharm, Jinan, Shandong, Peoples R China
[3] Shandong Univ, Sch Pharmaceut Sci, Dept Clin Pharm, Jinan, Shandong, Peoples R China
来源：
BIOMEDICAL CHROMATOGRAPHY | 2018年 / 32卷 / 04期
关键词：
cefoperazone; children; HPLC-MS; MS; sulbactam; BETA-LACTAM ANTIBIOTICS; HUMAN PLASMA; MASS-SPECTROMETRY; PHARMACOKINETICS; COMBINATION;
D O I：
10.1002/bmc.4143
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid, accurate and specific high-performance liquid chromatography-tandem mass spectrometry method has been validated for the simultaneous determination of cefoperazone and sulbactam in a small volume sample for children. A Shim-pack XR-ODS C-18 column with gradient elution of water (0.1% formic acid) and acetonitrile (0.1% formic acid) solution was used for separation at a flow rate of 0.3mL/min. The calibration curves of two analytes in serum showed excellent linearity over the concentration ranges of 0.03-10g/mL for cefoperazone, and 0.01-3g/mL for sulbactam, respectively. This method involves simple sample preparation steps and was validated according to standard US Food and Drug Administration and European Medicines Agency guidelines in terms of selectivity, linearity, detection limits, matrix effects, accuracy, precision, recovery and stability. This assay can be easily implemented in clinical practice to determine concentrations of cefoperazone and sulbactam in children.
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页数：6
相关论文
共 20 条
  • [1] Simultaneous determination of nine β-lactam antibiotics in human plasma by an ultrafast hydrophilic-interaction chromatography-tandem mass spectrometry
    Abdulla, Alan
    Bahmany, Soma
    Wijma, Rixt A.
    van der Nagel, Bart C. H.
    Koch, Birgit C. P.
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2017, 1060 : 138 - 143
  • [2] [Anonymous], 2001, Guidance for industry, bioanalytical method validation
  • [3] Development and validation of stability indicating HPLC methods for related substances and assay analyses of amoxicillin and potassium clavulanate mixtures
    Atici, Esen Bellur
    Yazar, Yucel
    Agtas, Cagan
    Ridvanoglu, Nurten
    Karliga, Bekir
    [J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2017, 136 : 1 - 9
  • [4] Quantification of seven β-lactam antibiotics and two β-lactamase inhibitors in human plasma using a validated UPLC-MS/MS method
    Carlier, Mieke
    Stove, Veronique
    Roberts, Jason A.
    Van de Velde, Eric
    De Waele, Jan J.
    Verstraete, Alain G.
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 2012, 40 (05) : 416 - 422
  • [5] CEFOPERAZONE PHARMACOKINETICS IN ACUTE CHILDHOOD MENINGITIS
    CHARTRAND, SA
    MARKS, MI
    JOHNSTON, JT
    FREDERICK, DF
    SCRIBNER, RK
    [J]. JOURNAL OF PEDIATRICS, 1983, 103 (06) : 991 - 995
  • [6] Stability, compatibility and microbiological activity studies of meropenem-clavulanate potassium
    Cielecka-Piontek, Judyta
    Szymanowska-Powalowska, Daria
    Paczkowska, Magdalena
    Lysakowski, Piotr
    Zalewski, Przemyslaw
    Garbacki, Piotr
    [J]. JOURNAL OF ANTIBIOTICS, 2015, 68 (01) : 35 - 39
  • [7] Clinical and Laboratory Standards Institute, 2016, PERFORMANCE STANDARD
  • [8] European Medicines Agency Committee for Medicinal Products for Human Use, 2012, GUID BIOAN METH VAL
  • [9] INVITRO ANTIMICROBIAL SPECTRUM, OCCURRENCE OF SYNERGY, AND RECOMMENDATIONS FOR DILUTION SUSCEPTIBILITY TESTING CONCENTRATIONS OF THE CEFOPERAZONE-SULBACTAM COMBINATION
    JONES, RN
    BARRY, AL
    PACKER, RR
    GREGORY, WW
    THORNSBERRY, C
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1987, 25 (09) : 1725 - 1729
  • [10] Standard line slopes as a measure of a relative matrix effect in quantitative HPLC-MS bioanalysis
    Matuszewski, BK
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2006, 830 (02): : 293 - 300
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