Helminth genome analysis reveals conservation of extracellular vesicle biogenesis pathways but divergence of RNA loading machinery between phyla

被引：16

作者：

Bennett, Adam P. S. ^{[1
]}

de la Torre-Escudero, Eduardo ^{[1
]}

Robinson, Mark W. ^{[1
]}

机构：

[1] Queens Univ Belfast, Sch Biol Sci, 19 Chlorine Gardens, Belfast, Antrim, North Ireland

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2020年 / 50卷 / 09期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Extracellular vesicle; Microvesicle; Biogenesis; Trematode; Nematode; RNA; PLASMA-MEMBRANE; PROTEIN; MECHANISMS;

D O I：

10.1016/j.ijpara.2020.04.004

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

A comparative genomics approach was used to determine whether established extracellular vesicle biogenesis pathways are conserved in helminths. This revealed conservation of membrane and cytoskeletal organising proteins as well as the endosomal sorting complex required for transport previously described in mammalian cells. Domain level analysis of this complex in helminths, however, indicated that some species may rely on atypical proteins to support subunit interactions and cargo recruitment. Interestingly, helminths displayed phylum level divergence of proteins associated with loading RNA into extracellular vesicles. These findings provide a framework for functional studies of helminth extracellular vesicle biogenesis and cargo sorting. (C) 2020 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.

引用

页码：655 / 661

页数：7

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